Detailed information of TA system TA154612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4244236..4245020
• Replicon: chromosome
• Accession: NZ_CP051652
• Organism: Pectobacterium carotovorum strain WPP14

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HER17_RS18910
• Protein ID: WP_029368386.1
• Coordinates: 4244236..4244730 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A855MHZ3
• Locus tag: HER17_RS18915
• Protein ID: WP_010301483.1
• Coordinates: 4244727..4245020 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HER17_RS18895	4239746..4242025	-	2280	WP_010301495.1	S8 family peptidase	-
HER17_RS18900	4242028..4243152	-	1125	WP_005970980.1	ATP-binding protein	-
HER17_RS18905	4244004..4244174	-	171	Protein_3695	helix-turn-helix domain-containing protein	-
HER17_RS18910	4244236..4244730	-	495	WP_029368386.1	GNAT family N-acetyltransferase	Toxin
HER17_RS18915	4244727..4245020	-	294	WP_010301483.1	DUF1778 domain-containing protein	Antitoxin
HER17_RS18920	4245349..4246251	-	903	WP_029368384.1	LysR family transcriptional regulator	-
HER17_RS18925	4246380..4246643	+	264	WP_010286385.1	DUF2798 domain-containing protein	-
HER17_RS18930	4246736..4247521	-	786	WP_180890064.1	phosphonate metabolism protein PhnP	-
HER17_RS18935	4247560..4248129	-	570	WP_161502897.1	ribose 1,5-bisphosphokinase	-
HER17_RS18940	4248248..4248460	-	213	Protein_3702	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HER17_RS18945	4248457..4248678	-	222	Protein_3703	ATP-binding cassette domain-containing protein	-
HER17_RS18950	4248754..4248891	+	138	WP_180890065.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17583.40 Da
• Isoelectric Point: 7.8050

>T154612 WP_029368386.1 NZ_CP051652:c4244730-4244236 [Pectobacterium carotovorum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARNFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T154612 NZ_CP068155:c4654267-4652945 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10930.52 Da
• Isoelectric Point: 10.1968

>AT154612 WP_010301483.1 NZ_CP051652:c4245020-4244727 [Pectobacterium carotovorum]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQTPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154612 NZ_CP068155:c4654533-4654267 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A855MHZ3