Detailed information of TA system TA154610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3772889..3773667
• Replicon: chromosome
• Accession: NZ_CP051652
• Organism: Pectobacterium carotovorum strain WPP14

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HER17_RS16735
• Protein ID: WP_029368127.1
• Coordinates: 3772889..3773377 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HER17_RS16740
• Protein ID: WP_010299613.1
• Coordinates: 3773374..3773667 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HER17_RS16710	3768030..3768200	-	171	WP_157757032.1	hypothetical protein	-
HER17_RS16715	3768342..3769088	+	747	WP_010299630.1	hypothetical protein	-
HER17_RS16720	3769440..3770801	+	1362	WP_029368129.1	hypothetical protein	-
HER17_RS16725	3771294..3771695	-	402	WP_010299624.1	hypothetical protein	-
HER17_RS16730	3771692..3772576	-	885	WP_010299620.1	ParA family protein	-
HER17_RS16735	3772889..3773377	-	489	WP_029368127.1	GNAT family N-acetyltransferase	Toxin
HER17_RS16740	3773374..3773667	-	294	WP_010299613.1	DUF1778 domain-containing protein	Antitoxin
HER17_RS16745	3774002..3774244	-	243	WP_010299611.1	helix-turn-helix domain-containing protein	-
HER17_RS16750	3774349..3774603	+	255	WP_049776261.1	helix-turn-helix domain-containing protein	-
HER17_RS16755	3774688..3774936	+	249	WP_010299606.1	helix-turn-helix domain-containing protein	-
HER17_RS16760	3774951..3775169	+	219	WP_010299595.1	type II toxin-antitoxin system antitoxin CcdA	-
HER17_RS16765	3775290..3775583	-	294	WP_010299593.1	DUF1778 domain-containing protein	-
HER17_RS16770	3775790..3776290	-	501	WP_010299591.1	serine acetyltransferase	-
HER17_RS16775	3776317..3777711	-	1395	WP_180890023.1	DUF4832 domain-containing protein	-
HER17_RS16780	3777750..3778157	-	408	WP_010303892.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3765173..3773918	8745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17508.32 Da
• Isoelectric Point: 6.8530

>T154610 WP_029368127.1 NZ_CP051652:c3773377-3772889 [Pectobacterium carotovorum]
MISAPEPLHAEHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGILVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVD
SL

Nucleotide

• Length: 489 bp

>T154610 NZ_CP068155:3268703-3269038 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10770.39 Da
• Isoelectric Point: 10.3968

>AT154610 WP_010299613.1 NZ_CP051652:c3773667-3773374 [Pectobacterium carotovorum]
MPATNSLSVKRETLNLRIKPAEGDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQKFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154610 NZ_CP068155:3268455-3268703 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA