Detailed information of TA system TA154590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 51035..51564
• Replicon: chromosome
• Accession: NZ_CP051643
• Organism: Staphylococcus caprae strain SY333

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C5QLA1
• Locus tag: HHJ99_RS00290
• Protein ID: WP_002445525.1
• Coordinates: 51202..51564 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HHJ99_RS00285
• Protein ID: WP_049401954.1
• Coordinates: 51035..51205 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ99_RS00260	46850..47329	+	480	WP_044466354.1	PH domain-containing protein	-
HHJ99_RS00265	47322..48821	+	1500	WP_044466353.1	PH domain-containing protein	-
HHJ99_RS00270	48808..49350	+	543	WP_044466352.1	PH domain-containing protein	-
HHJ99_RS00275	49365..49721	+	357	WP_044466351.1	holo-ACP synthase	-
HHJ99_RS00280	49799..50947	+	1149	WP_002445529.1	alanine racemase	-
HHJ99_RS00285	51035..51205	+	171	WP_049401954.1	hypothetical protein	Antitoxin
HHJ99_RS00290	51202..51564	+	363	WP_002445525.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HHJ99_RS00295	51936..52937	+	1002	WP_044466350.1	PP2C family protein-serine/threonine phosphatase	-
HHJ99_RS00300	53040..53366	+	327	WP_037541852.1	anti-sigma factor antagonist	-
HHJ99_RS00305	53368..53847	+	480	WP_002445522.1	anti-sigma B factor RsbW	-
HHJ99_RS00310	53822..54592	+	771	WP_002445521.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13511.68 Da
• Isoelectric Point: 10.0250

>T154590 WP_002445525.1 NZ_CP051643:51202-51564 [Staphylococcus caprae]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSESKMKEVDNALDISLGLHNFDHPKS

Nucleotide

• Length: 363 bp

>T154590 NZ_CP068152:c1471226-1470657 [Proteus mirabilis]
ATGACAAACTATACTGTTTTGCTTGATGCCTGTGTCATGTATCCAGCCCCTCTTAGAAGTTATTTAATGTATCTTGCTAA
TACTGGTTTATTTAGAGCACGTTGGAGTAATCAGATCCATGATGAATGGATCACTAATTTATTAGTCAACAATCCAACAT
TAACAGCTGAGCGACTAATACGAACCAGAGATTTAATGAATGCACATGTTCCTGATTGTTTAGTAGAAGGCTACGAACCG
CTTATTTGTGGTTTAAAGCTACCTGATCCTAATGATCGTCATGTGCTAGCAGCAGCAATAAAAGGGCAGGCTCAAGCTAT
AATTACATTTAACTTAAAAGATTTTCCGCAAAAATACCTTAATTCTTTTGATATTTCAGCTATCCATCCTGATGAATTTT
TGAGTGACATGTTTGAATTAAATCCTAGTAGCTGTATTAAAGCGGCGCAACAACATAGACAAAGCCTAAAGAATCCACCA
ATGACCGTAGAGACCTTTTTAGGCTGTTTACAAAAGCAGAAATTACCCCAGTTTATTTCCCATTTACGGGCATTCCAGAT
ATTATTATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6360.01 Da
• Isoelectric Point: 4.1929

>AT154590 WP_049401954.1 NZ_CP051643:51035-51205 [Staphylococcus caprae]
MLSFNQNRRHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNETYLTSNSKNE

Nucleotide

• Length: 171 bp

>AT154590 NZ_CP068152:c1471678-1471226 [Proteus mirabilis]
ATGAATACGATAAGTGAGAGACTTAGTGCTGAAGATATTGCATTAGCAAAATTAAGTAGCCAAGAGCTATCCGCTGTTAT
GGATATTAATGGCAAAACGCAGAAGCTATCCATTATAGATAAGACGGGTAATAATCATATTGTACAAATACCAAATAGTG
CATTACGCCTAATGATTGAAATTCTTACTCAACTAGGGCAAGGGAATACTGTTAGTATCACACCAATCCATGCTGAGCTA
ACGACACAGGAAGCGGCTGATATACTCAATATGTCCCGTCCTACATTTGTAAAATTATTAGATACACGAGCGATACCCTA
CTCTAGAAAAGGAAATCGTAGGAAAGTTGCTTATGCTGATATTGTTGCTTATAAAAATGAATTGGAGCAAAAACGACTTG
ATGCCCTTGCTGAATTATCGCAATTAGATCAAGAAATGGATATGGGGTATTAA

Structures

Toxin

• AlphaFold DB: A0A1S1GUI8