Detailed information of TA system TA154581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 266049..266636
• Replicon: chromosome
• Accession: NZ_CP051642
• Organism: Avibacterium paragallinarum strain ADL-AP01

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HHJ56_RS01345
• Protein ID: WP_136711940.1
• Coordinates: 266049..266345 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HHJ56_RS01350
• Protein ID: WP_017807324.1
• Coordinates: 266346..266636 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ56_RS01305	261560..262501	-	942	WP_035689276.1	hypothetical protein	-
HHJ56_RS01315	262716..263390	-	675	WP_051128138.1	hypothetical protein	-
HHJ56_RS01320	263380..263655	-	276	WP_035689243.1	hypothetical protein	-
HHJ56_RS01325	263677..263985	-	309	WP_046097548.1	hypothetical protein	-
HHJ56_RS01330	264079..264651	-	573	WP_168952571.1	hypothetical protein	-
HHJ56_RS01335	265324..265545	-	222	WP_017806446.1	hypothetical protein	-
HHJ56_RS01340	265560..265730	+	171	WP_017807323.1	DUF1508 domain-containing protein	-
HHJ56_RS01345	266049..266345	+	297	WP_136711940.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ56_RS01350	266346..266636	+	291	WP_017807324.1	putative addiction module antidote protein	Antitoxin
HHJ56_RS01355	266783..267556	-	774	WP_017807325.1	DUF1828 domain-containing protein	-
HHJ56_RS01360	267558..268043	-	486	WP_017807326.1	hypothetical protein	-
HHJ56_RS01365	268043..268696	-	654	WP_017807327.1	LexA family transcriptional regulator	-
HHJ56_RS01370	268834..269040	+	207	WP_017807328.1	helix-turn-helix transcriptional regulator	-
HHJ56_RS01375	269088..269528	+	441	Protein_265	hypothetical protein	-
HHJ56_RS01380	269546..269953	-	408	WP_168939907.1	hypothetical protein	-
HHJ56_RS01385	270063..270833	+	771	WP_046097539.1	phage regulatory protein/antirepressor Ant	-
HHJ56_RS01390	270830..271636	+	807	WP_035689420.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	244535..292406	47871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11040.10 Da
• Isoelectric Point: 10.5997

>T154581 WP_136711940.1 NZ_CP051642:266049-266345 [Avibacterium paragallinarum]
MIQIKSTTLFKKWLDELKDLRARAKIQTRIKRLQLGNFGDVKPVGEGISELRITEGKGYRIYLKNQNGVVVILLCAGDKS
IQSKDIEKAKLLAKELGV

Nucleotide

• Length: 297 bp

>T154581 NZ_CP068151:2522098-2522412 [Pseudomonas fluorescens]
ATGATTTTCATTGAGACATCGGTCTTCACACGTCGCGTTAAAGAATTGATCAATGAAGATGCCTACACGGCCTTTCAGAA
CGTGCTTGTAGTGAATCCCGCTGCAGGCGATGTGATTGAGGGTACGGGCGGTATCCGCAAAATACGAGTAGCAGCCAAGA
GCCATGGTAAACGAGGCGGGGCACGTGTGATCTATTACCACTTCGCCTCGGCATCACAGATTGTTTTACTGATGATCTAT
CCCAAAAATGAACAGCAGGACTTGAGCGCCGACGAACGCAAGTCACTGAAGGCTGCGATTGAGCACTGGAGATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10812.39 Da
• Isoelectric Point: 4.8550

>AT154581 WP_017807324.1 NZ_CP051642:266346-266636 [Avibacterium paragallinarum]
MMELQDFDIAEHLRSEEEIQLYLNEVLQENDVDLILSALGDIAKARNMSQLAREVGVSREGLYKAFSGKGNPTFSTVLKV
MKALNLSFEVKRVTHA

Nucleotide

• Length: 291 bp

>AT154581 NZ_CP068151:2522415-2522702 [Pseudomonas fluorescens]
ATGAGCCGACTTTTTGATGAATTGATGGAAAGCGTTCAAGAGATGAACGAGATCCTGCGCGGAGAACGGGAGCCATCCCG
TGAATTTCATGTCGACTCAATGCGAGTTAAAGAGATCCGTAAGGCAACAGGGCTGACCCAGGCTAAATTCGCAGCGCTGA
TTGACGTTCAGCTCGGTACGTTGCGCAACTGGGAGCAGGGTCGTCGCGAACCTACTGGGCCGGCAAAGGCGTTGTTGAAA
GCTATCCATAACGACCCGGTACATGTACTGAACGCGCTGGCTAGCTAA