Detailed information of TA system TA154576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 693418..695051
• Replicon: chromosome
• Accession: NZ_CP051641
• Organism: Avibacterium paragallinarum strain ADL-AP02

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A3G8W245
• Locus tag: HHJ57_RS03330
• Protein ID: WP_035684858.1
• Coordinates: 693744..695051 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HHJ57_RS03325
• Protein ID: WP_081637498.1
• Coordinates: 693418..693744 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ57_RS03305	688920..689651	+	732	WP_035684848.1	class I SAM-dependent methyltransferase	-
HHJ57_RS03310	689758..691527	+	1770	WP_035684851.1	30S ribosomal protein S12 methylthiotransferase accessory factor YcaO	-
HHJ57_RS03315	691662..692084	+	423	WP_035684853.1	DUF1043 family protein	-
HHJ57_RS03320	692247..693377	+	1131	WP_035684855.1	ribonucleotide-diphosphate reductase subunit beta	-
HHJ57_RS03325	693418..693744	-	327	WP_081637498.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ57_RS03330	693744..695051	-	1308	WP_035684858.1	HipA domain-containing protein	Toxin
HHJ57_RS03335	695151..695399	-	249	WP_035684861.1	2Fe-2S ferredoxin-like protein	-
HHJ57_RS03340	696674..697486	-	813	WP_035684866.1	4-hydroxy-tetrahydrodipicolinate reductase	-
HHJ57_RS03345	697501..698136	-	636	WP_035684869.1	LysE family transporter	-
HHJ57_RS03350	698148..698648	-	501	WP_017806513.1	phosphatidylglycerophosphatase A	-
HHJ57_RS03355	698654..699688	-	1035	WP_035684872.1	thiamine-phosphate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 50363.28 Da
• Isoelectric Point: 5.6627

>T154576 WP_035684858.1 NZ_CP051641:c695051-693744 [Avibacterium paragallinarum]
MEKLTLQTYIDQEWRDMAELHFEKETYQLSNLVYFPDYITQYFGQEGSRAVSINYPVHIFIENRKTLWSCIGDIAPAGAS
RRYWIESLDIKHLPESEQNYRLLKYGAISPVGNLRIKEATPQKTEVRYFDIEKVKDRQSDFLEYANQSGAMVGGATGAGG
EAPKLLLKRHNGKVWIDNLQDGKDQDISYLVKYPRGRYSPIDCDILRAEFHYYQEVAELGFDTIDIHHMCLEEGERYPSL
WLPRFDITKKEGKYNRLALESVYSMLQKQGGTLEHEVTIRQLIKIISASNLEFDQQHFVLEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQKIWLAPIYDFAPMRADPEGIIRSTTWNRKGSLPQEFAGEYRFDLIAESLSDLIEPSLLLTELQQLAIRLVD
LKSRLAQRGVPQAILDHPSIGFDYLPEKLKAWGLV

Nucleotide

• Length: 1308 bp

>T154576 NZ_CP068148:c4654713-4653415 [Serratia liquefaciens]
ATGGAAAAGCTACGCGTGGCGCTAAATGGTACTTTGGTTGGAATGCTGGAGAAGCATGCAGGCGGCGTGATGTCGTTTGT
TTATCAGGAAGAGTGGCTGGCACAAGCTGGCTCGCGTGCCATATCGTTATCCCTACCGTTACAACCAGAGCCGTTTCGCG
GTGACGTGGTATATAACTTTTTCGACAACCTGCTGCCGGACAGCGAGCTTATTCGCAGCCGCATACAGGCGCGTTTTAGA
ACGGTAACCAAACAACCTTTTGATCTTCTGTCCAGCATTGGTAGCGACTGCGTGGGGGCGATCCAGCTTTACCCTGAACA
TGCTGAGGTGGCCCCGGTAAAGCAAGTGAATGCAGTACCGATCAGTGACGAAGAAATAGAGCAGTTACTGGAAGGCTACC
GCGAAGCCCCCTTGGGCATGGGACCAGATATTGATGATTTTCGAATATCTCTGGCAGGCGCGCAGGAAAAGACGGCTCTG
CTTTGGTTCGACAATCAGTGGTACCGACCTGAAGGAAGCACACCGACCAGTCACATTCTAAAACTGCCCATCGGCCATAT
CGCGCATAACAATTTGGATCTCAGCGAAAGCTGTGAGAACGAGTGGTTATGTTTACGCATGGCTGAACAGTTTGGCCTGC
CGGTAGCACAAACTCAGTTAGCGCATTTTGGTGAGCAGAAGGTCCTGATCGTCGAGCGCTTTGACCGACGTTGGGCGCGG
GATAATAGCTGGCTGATTCGCTTGCCGCAGGAGGATTTTTGCCAGGCGTTAGGCATTGCTCCTGCGCTGAAATACGAGGC
TGATGGCGGGCCCGGTATTACGGCTGCTATGGAATTGCTATTGGGCTCCCAACAGCCCTTGCTCGATCGAGACACTTTCT
TTAAAAGCCAAATCGTATTCTGGATGCTGGCGGCCATTGATGGGCATGCTAAGAACTTTAGTCTGTTCCTTCAGCCGGAA
ACGACTTATCGAATGACCCCACTTTATGATGTGCTCTCGGGTTACCCCTTTATGCACGCCCGCGGCATCGCACCGCAGAA
AGCAAAAATGGCCATGGCTGCGATTGGGAAGGGCCGCCATTACCGTTGGAGCCAGATCTTGCCACGGCACTTTATCTCTA
CCGCGAGAGCTGTGGGGTATTCTGAAGAGAAAACGCTTCAGGTGATGGCTGAGTTGAAAGCGCAAACGGATCAAGCGATC
GCCAATGTGGAAGCGATGCTGCCGGATGATTTTCCCCGCTTTGTTCGTGATGCTATTTTTGCCGGGGTTCGGCGGCAAGC
GGAGAGATTGCCGGGGTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12252.39 Da
• Isoelectric Point: 10.9810

>AT154576 WP_081637498.1 NZ_CP051641:c693744-693418 [Avibacterium paragallinarum]
MITRKNASKVGATISTTERNNVRNRIIKALLLGKMTQGQALRKLRIEMLGVNQQQYMKVVKVSRQTLSNIENDKGNYSVE
TINQVFKPFGFELGLVPISKELLTEIMQ

Nucleotide

• Length: 327 bp

>AT154576 NZ_CP068148:c4654964-4654716 [Serratia liquefaciens]
ATGAAAATCACCTCGGCACAGATGCTGGCGCATACATTACGCAATGAGCGTAAGAAGCGTAACCAAAGCCAAAGTAAAAC
TGCTGACAGCGTTGGGTTAAAACAGGCGACGATTTCCGCGTTTGAAAACAACCCGGACGGCACCCGGCTGGAGACGTTGT
TCAAACTACTTGCTGCGCTGGACTTGGAACTGCAGGTCACCCCGCGTGGCAAGCCGGTAGATCCCAAGACCTGGGATCAG
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3G8W245