Detailed information of TA system TA154557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 265996..266583
• Replicon: chromosome
• Accession: NZ_CP051639
• Organism: Avibacterium paragallinarum strain ADL-AP10

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HHJ59_RS01340
• Protein ID: WP_136711940.1
• Coordinates: 265996..266292 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HHJ59_RS01345
• Protein ID: WP_017807324.1
• Coordinates: 266293..266583 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ59_RS01300	261435..262376	-	942	WP_035689276.1	hypothetical protein	-
HHJ59_RS01310	262660..262839	-	180	Protein_253	hypothetical protein	-
HHJ59_RS01315	263257..263532	-	276	WP_035689243.1	hypothetical protein	-
HHJ59_RS01320	263640..263861	-	222	WP_168985396.1	hypothetical protein	-
HHJ59_RS01325	263955..264619	-	665	Protein_256	polymer-forming cytoskeletal protein	-
HHJ59_RS01330	265271..265492	-	222	WP_017806446.1	hypothetical protein	-
HHJ59_RS01335	265507..265677	+	171	WP_017807323.1	DUF1508 domain-containing protein	-
HHJ59_RS01340	265996..266292	+	297	WP_136711940.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HHJ59_RS01345	266293..266583	+	291	WP_017807324.1	putative addiction module antidote protein	Antitoxin
HHJ59_RS01350	266730..267503	-	774	WP_017807325.1	DUF1828 domain-containing protein	-
HHJ59_RS01355	267505..267990	-	486	WP_017807326.1	hypothetical protein	-
HHJ59_RS01360	267990..268643	-	654	WP_017807327.1	LexA family transcriptional regulator	-
HHJ59_RS01365	268781..268987	+	207	WP_017807328.1	helix-turn-helix transcriptional regulator	-
HHJ59_RS01370	269035..269475	+	441	Protein_265	hypothetical protein	-
HHJ59_RS01375	269493..269900	-	408	WP_168939907.1	hypothetical protein	-
HHJ59_RS01380	270010..270780	+	771	WP_046097539.1	phage regulatory protein/antirepressor Ant	-
HHJ59_RS01385	270777..271583	+	807	WP_035689420.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	244412..292353	47941

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11040.10 Da
• Isoelectric Point: 10.5997

>T154557 WP_136711940.1 NZ_CP051639:265996-266292 [Avibacterium paragallinarum]
MIQIKSTTLFKKWLDELKDLRARAKIQTRIKRLQLGNFGDVKPVGEGISELRITEGKGYRIYLKNQNGVVVILLCAGDKS
IQSKDIEKAKLLAKELGV

Nucleotide

• Length: 297 bp

>T154557 NZ_CP068147:2033818-2034069 [Yersinia enterocolitica]
ATGAGAACAATTAGTTATAGTGAGGCGCGCCAGAATTTGTCGACAACGATGGTGCAAACGGTTGAGGATCGAGCCCCCAT
CCTCATCACTCGTCAAAATGGGACTTCTTGTGTTCTTATGTCACTTGAAGAATATGAATCATTGGAAGAAACTGCTTATT
TACTGCGCTCACCAGCAAACGCGAAGCATTTGATGGACTCAATTGAAGAGTTGAGAGCAGGAAAAGGAATTCAAAGGGAA
CTTGAAGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10812.39 Da
• Isoelectric Point: 4.8550

>AT154557 WP_017807324.1 NZ_CP051639:266293-266583 [Avibacterium paragallinarum]
MMELQDFDIAEHLRSEEEIQLYLNEVLQENDVDLILSALGDIAKARNMSQLAREVGVSREGLYKAFSGKGNPTFSTVLKV
MKALNLSFEVKRVTHA

Nucleotide

• Length: 291 bp

>AT154557 NZ_CP068147:2034066-2034320 [Yersinia enterocolitica]
GTGAGAATTATATTTTCCAGTTGTTCTTGGGAGGATTATCTTTATTGGCAACAAACGGATAAGAAAATCCTCAAACGCAT
TAATGAGTTAGTAAAAAATATTCAAAGAACGCCATTTGAGGGAAAGGGTAAACCGGAACCCCTTAAACATAATCTGGCAG
GGTTCTGGTCACGGAGGATTACAGAAGAGCACAGACTTGTTTATGAGGTTTCCGGTGATAATTTATTAATTGCTGCTTGT
CGTTACCATTATTGA