Detailed information of TA system TA154529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1991736..1992301
• Replicon: chromosome
• Accession: NZ_CP051635
• Organism: Treponema denticola strain OMZ 905

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M2BU34
• Locus tag: E4N86_RS09200
• Protein ID: WP_010694033.1
• Coordinates: 1991963..1992301 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E4N86_RS09195
• Protein ID: WP_253699002.1
• Coordinates: 1991736..1991969 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N86_RS09165 (E4N86_09190)	1987429..1987590	-	162	WP_253718311.1	hypothetical protein	-
E4N86_RS09170 (E4N86_09195)	1987635..1990010	+	2376	WP_253717722.1	endopeptidase La	-
E4N86_RS09175 (E4N86_09200)	1990094..1990321	+	228	WP_253698999.1	DUF2281 domain-containing protein	-
E4N86_RS09180 (E4N86_09205)	1990318..1990545	+	228	WP_253699000.1	PIN domain-containing protein	-
E4N86_RS09185 (E4N86_09210)	1990595..1990771	+	177	WP_253717718.1	hypothetical protein	-
E4N86_RS09190 (E4N86_09215)	1990884..1991261	+	378	WP_253717716.1	hypothetical protein	-
E4N86_RS09195 (E4N86_09220)	1991736..1991969	+	234	WP_253699002.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4N86_RS09200 (E4N86_09225)	1991963..1992301	+	339	WP_010694033.1	endoribonuclease MazF	Toxin
E4N86_RS09205 (E4N86_09230)	1992404..1993246	+	843	WP_253717714.1	hypothetical protein	-
E4N86_RS09210 (E4N86_09235)	1993407..1994450	+	1044	WP_253699005.1	DMT family transporter	-
E4N86_RS09215 (E4N86_09240)	1994578..1995393	+	816	WP_253699006.1	protein-ADP-ribose hydrolase	-
E4N86_RS09220 (E4N86_09245)	1995411..1996280	+	870	WP_253699007.1	Sir2 silent information regulator family NAD-dependent deacetylase	-
E4N86_RS09225 (E4N86_09250)	1996289..1996708	+	420	WP_253699008.1	Rrf2 family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12821.66 Da
• Isoelectric Point: 6.6952

>T154529 WP_010694033.1 NZ_CP051635:1991963-1992301 [Treponema denticola]
MVNSNYVPDRGDLVWLDFNPQAGHEQKGRRPAICISQKRYNQKVGLALFCPITSHIKGYPFEIVLDNHSINGCILSDQVK
NLDYKKRSCDFIEKTTEEEINAVVDNIKLLIE

Nucleotide

• Length: 339 bp

>T154529 NZ_CP068143:676218-676580 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8624.85 Da
• Isoelectric Point: 5.6423

>AT154529 WP_253699002.1 NZ_CP051635:1991736-1991969 [Treponema denticola]
MQVVVQKWGNSLGFRIPSLWAKDNNVKHGSKIEVIAEKGKIVILPQEKNLDDMLAMVTSENVHSEISTGYSVGNEEW

Nucleotide

• Length: 234 bp

>AT154529 NZ_CP068143:676051-676221 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: M2BU34