Detailed information of TA system TA154526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1216258..1216928
• Replicon: chromosome
• Accession: NZ_CP051635
• Organism: Treponema denticola strain OMZ 905

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E4N86_RS05550
• Protein ID: WP_253716149.1
• Coordinates: 1216258..1216617 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: E4N86_RS05555
• Protein ID: WP_253700941.1
• Coordinates: 1216614..1216928 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N86_RS05520 (E4N86_05545)	1211679..1212431	-	753	WP_253716154.1	ABC transporter ATP-binding protein	-
E4N86_RS05525 (E4N86_05550)	1213013..1213312	-	300	WP_253716153.1	HigA family addiction module antitoxin	-
E4N86_RS05530 (E4N86_05555)	1213326..1213607	-	282	WP_253716152.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4N86_RS05535 (E4N86_05560)	1213820..1214263	+	444	WP_253700937.1	hypothetical protein	-
E4N86_RS05540 (E4N86_05565)	1214285..1214824	+	540	WP_253716151.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
E4N86_RS05545 (E4N86_05570)	1214975..1216258	+	1284	WP_253716150.1	family 1 glycosylhydrolase	-
E4N86_RS05550 (E4N86_05575)	1216258..1216617	+	360	WP_253716149.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4N86_RS05555 (E4N86_05580)	1216614..1216928	+	315	WP_253700941.1	putative addiction module antidote protein	Antitoxin
E4N86_RS05560 (E4N86_05585)	1216965..1217906	-	942	WP_253716148.1	DMT family transporter	-
E4N86_RS05565 (E4N86_05590)	1217936..1219078	-	1143	WP_253716147.1	hypothetical protein	-
E4N86_RS05570 (E4N86_05595)	1219143..1220549	-	1407	WP_253716146.1	tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB	-
E4N86_RS05575 (E4N86_05600)	1220553..1221194	-	642	WP_253700945.1	tetratricopeptide repeat protein	-
E4N86_RS05580 (E4N86_05605)	1221184..1221561	-	378	WP_253700946.1	polymer-forming cytoskeletal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (30-109)

Antitoxin

• Uncharacterized protein HI1420 (2-93)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14060.42 Da
• Isoelectric Point: 10.1762

>T154526 WP_253716149.1 NZ_CP051635:1216258-1216617 [Treponema denticola]
MNYNRLDSLLFLNYNIDMRTIKKTEVFDTWLSKLKDEKGKAKITDRIMRLKRGNKGDHRIIDKDIYELRIHFGPGYRIYC
TDKNNEIILLLIAGDKSTQPKDIKKAQQMIKLLEQGDTK

Nucleotide

• Length: 360 bp

>T154526 NZ_CP068143:343509-343775 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11790.35 Da
• Isoelectric Point: 5.1199

>AT154526 WP_253700941.1 NZ_CP051635:1216614-1216928 [Treponema denticola]
MKITDFDPADYLNTKEAMIEYLNEALKTSIEDNTPEHFTEALGDITRAQGFTNVAKHTDLNREHLYRSLSKKGNPTFSTI
LKLLNFLGLEINIIDPQTAKPAYC

Nucleotide

• Length: 315 bp

>AT154526 NZ_CP068143:343258-343509 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA