Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 115523..116048 Replicon plasmid pAPHA_2017_1
Accession NZ_CP051616
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HHM36_RS24700 Protein ID WP_001159868.1
Coordinates 115523..115828 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHM36_RS24705 Protein ID WP_000813634.1
Coordinates 115830..116048 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS24685 111433..112599 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HHM36_RS24690 113187..113942 - 756 WP_000852146.1 replication initiation protein RepE -
HHM36_RS24695 114716..115522 - 807 WP_000016982.1 site-specific integrase -
HHM36_RS24700 115523..115828 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HHM36_RS24705 115830..116048 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHM36_RS24710 116756..117751 + 996 WP_000246636.1 hypothetical protein -
HHM36_RS24715 117755..118687 + 933 WP_000991832.1 hypothetical protein -
HHM36_RS24720 118824..119521 - 698 Protein_140 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..134872 134872
- flank IS/Tn - - 118824..119327 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T154505 WP_001159868.1 NZ_CP051616:c115828-115523 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154505 NZ_CP068122:1119522-1119863 [Enterococcus hirae]
GTGCCTAAAGCTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGAAAT
TCAAAAGAGAAGACCAGGCTTGGTTGTATCTCGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTGTCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT154505 WP_000813634.1 NZ_CP051616:c116048-115830 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154505 NZ_CP068122:1119292-1119522 [Enterococcus hirae]
ATGCTCATTACAAAATCTAGATTACAAGGTAGTTCTGTAGTAATTACACTACCGTCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References