Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4065640..4066258 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HHM36_RS19320 Protein ID WP_001291435.1
Coordinates 4065640..4065858 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HHM36_RS19325 Protein ID WP_000344800.1
Coordinates 4065884..4066258 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS19285 4060927..4061499 + 573 WP_000779831.1 YbaY family lipoprotein -
HHM36_RS19290 4061530..4061841 - 312 WP_000409908.1 MGMT family protein -
HHM36_RS19300 4062220..4062573 + 354 WP_000878135.1 DUF1428 family protein -
HHM36_RS19305 4062615..4064165 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HHM36_RS19310 4064329..4064799 - 471 WP_000136192.1 YlaC family protein -
HHM36_RS19315 4064915..4065466 - 552 WP_000102539.1 maltose O-acetyltransferase -
HHM36_RS19320 4065640..4065858 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HHM36_RS19325 4065884..4066258 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HHM36_RS19330 4066804..4069953 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
HHM36_RS19335 4069976..4071169 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T154491 WP_001291435.1 NZ_CP051615:c4065858-4065640 [Escherichia coli O25b:H4-ST131]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T154491 NZ_CP068102:c560245-559991 [Rothia aeria]
ATGAAAACCATGTCTTACACCGAGTCTCGGGCTCGGTATGCTGAAGTACTTAACCACGTTGAGGACCACCGCGAAGAGGT
TGTTATTACTCGATCGGGGCACGAGCCGGCAGTTATACTCTCCTTGTCTGAATATGAATCTCTTCAAGAGACTGCCTACC
TTATGCGTTCCCCTGCTAATGCACGTCGTCTTATGGAATCCATCTCTCGTCTTGAAGCTGGTCTTGGGGCTGAACATCAG
CTGATTGAGGAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT154491 WP_000344800.1 NZ_CP051615:c4066258-4065884 [Escherichia coli O25b:H4-ST131]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT154491 NZ_CP068102:c559988-559728 [Rothia aeria]
GTGAGAATTATTTTTGACGATTCAGCGTGGAGTGACTATCTCTGGTGGCAGGTGCAAGATCGGAAGGTTCTCAAGCGCAT
TAATCTACTGATCAAAGATGTTGAACGAAATGGTAATGAAGGTATAGGGAAGCCTGAAGCTCTCAGACATCAATTTTCAG
GCTGGTGGTCACGCAGAATTACTGATGAACACCGTCTCGTATACAAGCTTACTGACGATTCTATTGTCATAGCTTCATGC
CGATACCACTATGGGGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References