Detailed information of TA system TA154490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3881297..3882986
• Replicon: chromosome
• Accession: NZ_CP051615
• Organism: Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: HHM36_RS18460
• Protein ID: WP_000365807.1
• Coordinates: 3882066..3882986 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HHM36_RS18455
• Protein ID: WP_001295795.1
• Coordinates: 3881297..3882079 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM36_RS18450	3877915..3881013	-	3099	WP_000673070.1	type I restriction endonuclease subunit R	-
HHM36_RS18455	3881297..3882079	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HHM36_RS18460	3882066..3882986	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HHM36_RS18465	3883151..3884470	-	1320	Protein_3604	site-specific integrase	-
HHM36_RS18470	3884697..3884855	-	159	Protein_3605	integrase	-
HHM36_RS18475	3884904..3885089	+	186	WP_000146240.1	hypothetical protein	-
HHM36_RS18480	3885304..3885918	+	615	WP_001019923.1	YagU family protein	-
HHM36_RS18485	3885992..3886155	+	164	Protein_3608	tail fiber assembly protein	-
HHM36_RS18490	3886167..3886496	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
HHM36_RS18495	3886803..3887513	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	3875047..3923202	48155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T154490 WP_000365807.1 NZ_CP051615:3882066-3882986 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T154490 NZ_CP068102:c559988-559728 [Rothia aeria]
GTGAGAATTATTTTTGACGATTCAGCGTGGAGTGACTATCTCTGGTGGCAGGTGCAAGATCGGAAGGTTCTCAAGCGCAT
TAATCTACTGATCAAAGATGTTGAACGAAATGGTAATGAAGGTATAGGGAAGCCTGAAGCTCTCAGACATCAATTTTCAG
GCTGGTGGTCACGCAGAATTACTGATGAACACCGTCTCGTATACAAGCTTACTGACGATTCTATTGTCATAGCTTCATGC
CGATACCACTATGGGGTATAG

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT154490 WP_001295795.1 NZ_CP051615:3881297-3882079 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT154490 NZ_CP068102:c560245-559991 [Rothia aeria]
ATGAAAACCATGTCTTACACCGAGTCTCGGGCTCGGTATGCTGAAGTACTTAACCACGTTGAGGACCACCGCGAAGAGGT
TGTTATTACTCGATCGGGGCACGAGCCGGCAGTTATACTCTCCTTGTCTGAATATGAATCTCTTCAAGAGACTGCCTACC
TTATGCGTTCCCCTGCTAATGCACGTCGTCTTATGGAATCCATCTCTCGTCTTGAAGCTGGTCTTGGGGCTGAACATCAG
CTGATTGAGGAATAG

Structures

Toxin

• AlphaFold DB: A0A061KTE0