Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3221206..3221703 Replicon chromosome
Accession NZ_CP051615
Organism Escherichia coli O25b:H4-ST131 strain 2017_APHA

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHM36_RS15245 Protein ID WP_001173343.1
Coordinates 3221530..3221703 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHM36_RS15240 Protein ID WP_001312331.1
Coordinates 3221206..3221502 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM36_RS15230 3216563..3220492 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
HHM36_RS15235 3220706..3220978 + 273 WP_000405647.1 N-acetyltransferase -
HHM36_RS15240 3221206..3221502 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHM36_RS15245 3221530..3221703 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHM36_RS15250 3221822..3223339 - 1518 WP_000003806.1 lysine--tRNA ligase -
HHM36_RS15255 3223576..3225033 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3204838..3230556 25718


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154482 WP_001173343.1 NZ_CP051615:3221530-3221703 [Escherichia coli O25b:H4-ST131]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154482 NZ_CP068093:c3886-3620 [Shigella boydii]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154482 WP_001312331.1 NZ_CP051615:3221206-3221502 [Escherichia coli O25b:H4-ST131]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154482 NZ_CP068093:c4094-3870 [Shigella boydii]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References