Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 49538..50139 Replicon plasmid pAPHA_2019
Accession NZ_CP051610
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag HHM37_RS24715 Protein ID WP_001216034.1
Coordinates 49759..50139 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag HHM37_RS24710 Protein ID WP_001190712.1
Coordinates 49538..49759 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS24700 46531..47802 - 1272 WP_023142242.1 restriction endonuclease subunit S -
HHM37_RS24705 47799..49355 - 1557 WP_001617892.1 type I restriction-modification system subunit M -
HHM37_RS24710 49538..49759 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HHM37_RS24715 49759..50139 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HHM37_RS24720 50144..50323 + 180 WP_001513661.1 hypothetical protein -
HHM37_RS24725 50351..50710 + 360 WP_001513660.1 hypothetical protein -
HHM37_RS24730 50634..51047 + 414 Protein_61 DDE-type integrase/transposase/recombinase -
HHM37_RS24735 50997..51314 - 318 WP_001513659.1 hypothetical protein -
HHM37_RS24740 51542..52558 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
HHM37_RS24745 52766..54169 + 1404 WP_001373486.1 S-methylmethionine permease -
HHM37_RS24750 54156..55088 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..113351 113351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T154462 WP_001216034.1 NZ_CP051610:49759-50139 [Escherichia coli O25b:H4-ST131]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T154462 NZ_CP068073:c1222093-1221887 [Staphylococcus condimenti]
ATGCAAAACAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTACATCAACATCTTATAGAAAT
GACAGGTGTAACTGCAGTACAAGTAGATTTGGATTCAAATGAAATCAAAATCAGTTACGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCGGGTTATCAAATATTAAATTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT154462 WP_001190712.1 NZ_CP051610:49538-49759 [Escherichia coli O25b:H4-ST131]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT154462 NZ_CP068073:c1222398-1222105 [Staphylococcus condimenti]
ATGACCGAAAATGACAAAGCACATCATTCAGAACAAATTAAGTCCAACTTGAAAGCAAGACTCAACCGTATTGAGGGGCA
AGTCAGAGCAATTAACCGAATGATTGAAGAAGATGTTTATTGTGATGATGTACTTACACAAATCCGTGCAACACGTTCAG
CCTTGAACAGTGTAGCAGTACGATTGCTTGATCACCATATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCAATGGAAGAATTGCTTGTTACCGTTCAAAAATTAATTAAAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References