Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39081..39606 Replicon plasmid pAPHA_2019
Accession NZ_CP051610
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag HHM37_RS24670 Protein ID WP_001159868.1
Coordinates 39081..39386 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag HHM37_RS24675 Protein ID WP_000813634.1
Coordinates 39388..39606 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS24655 34991..36157 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
HHM37_RS24660 36745..37500 - 756 WP_000852146.1 replication initiation protein RepE -
HHM37_RS24665 38274..39080 - 807 WP_000016982.1 site-specific integrase -
HHM37_RS24670 39081..39386 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
HHM37_RS24675 39388..39606 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HHM37_RS24680 40314..41309 + 996 WP_000246636.1 hypothetical protein -
HHM37_RS24685 41313..42245 + 933 WP_000991832.1 hypothetical protein -
HHM37_RS24690 42382..43079 - 698 Protein_53 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / dfrA17 senB 1..113351 113351
- flank IS/Tn - - 42382..42759 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T154461 WP_001159868.1 NZ_CP051610:c39386-39081 [Escherichia coli O25b:H4-ST131]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154461 NZ_CP068073:c1208887-1208537 [Staphylococcus condimenti]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCGCCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAACGACACTGGAAATAAGTATAGTCCAACAGTCATTGTAGCTGCAATGACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCAGTTATTTTACTAGAACAGATAAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT154461 WP_000813634.1 NZ_CP051610:c39606-39388 [Escherichia coli O25b:H4-ST131]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154461 NZ_CP068073:c1209051-1208884 [Staphylococcus condimenti]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTCGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References