Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4149285..4149903 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HHM37_RS19755 Protein ID WP_001291435.1
Coordinates 4149285..4149503 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HHM37_RS19760 Protein ID WP_000344800.1
Coordinates 4149529..4149903 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS19720 4144572..4145144 + 573 WP_000779831.1 YbaY family lipoprotein -
HHM37_RS19725 4145175..4145486 - 312 WP_000409908.1 MGMT family protein -
HHM37_RS19735 4145865..4146218 + 354 WP_000878135.1 DUF1428 family protein -
HHM37_RS19740 4146260..4147810 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HHM37_RS19745 4147974..4148444 - 471 WP_000136192.1 YlaC family protein -
HHM37_RS19750 4148560..4149111 - 552 WP_000102539.1 maltose O-acetyltransferase -
HHM37_RS19755 4149285..4149503 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HHM37_RS19760 4149529..4149903 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HHM37_RS19765 4150449..4153598 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
HHM37_RS19770 4153621..4154814 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T154455 WP_001291435.1 NZ_CP051609:c4149503-4149285 [Escherichia coli O25b:H4-ST131]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T154455 NZ_CP068063:398945-399307 [Staphylococcus equorum]
ATGATGAGAAGAGGAGACGTTTATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
TATCATACAGAATGATACTGGAAATAAATATAGTCCTACCGTAATTGTAGCTGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAGCTTGATAAAGATTCTGTAATACTGCTTGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACATACCTTTCTGAAAGTAAAATGAAAGAAGTTAATGTTGCCATAGA
TATAAGCTTAGGATTACATAATGTTAGAAATAGTAAATCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT154455 WP_000344800.1 NZ_CP051609:c4149903-4149529 [Escherichia coli O25b:H4-ST131]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT154455 NZ_CP068063:398778-398948 [Staphylococcus equorum]
ATGGCAAGTTTTAATCAAAGCAGAAACCATAGTATTGAACAACTATTAAAAGAAGGCTATTCACAAATGGCCGATTTAAA
TCTCTCCCTTGCAGCAGAAGCATTTCCATTTGAATGTGAAGCTTGTGATTGCAATGAAGTTTACCTCAGCTCTAAGAATA
ACAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References