Detailed information of TA system TA154454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3963360..3965049
• Replicon: chromosome
• Accession: NZ_CP051609
• Organism: Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: HHM37_RS18890
• Protein ID: WP_000365807.1
• Coordinates: 3964129..3965049 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HHM37_RS18885
• Protein ID: WP_001295795.1
• Coordinates: 3963360..3964142 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM37_RS18880	3959978..3963076	-	3099	WP_000673070.1	type I restriction endonuclease subunit R	-
HHM37_RS18885	3963360..3964142	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HHM37_RS18890	3964129..3965049	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HHM37_RS18895	3965214..3966533	-	1320	Protein_3688	site-specific integrase	-
HHM37_RS18900	3966760..3966918	-	159	Protein_3689	integrase	-
HHM37_RS18905	3966967..3967152	+	186	WP_000146240.1	hypothetical protein	-
HHM37_RS18910	3967367..3967981	+	615	WP_001019923.1	YagU family protein	-
HHM37_RS18915	3968055..3968218	+	164	Protein_3692	tail fiber assembly protein	-
HHM37_RS18920	3968230..3968559	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
HHM37_RS18925	3968866..3969576	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T154454 WP_000365807.1 NZ_CP051609:3964129-3965049 [Escherichia coli O25b:H4-ST131]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T154454 NZ_CP068063:387612-387824 [Staphylococcus equorum]
GTGATAACAAAAGTTATTTATATTTCCGGTATAGAAAATGAAACACAACAAAAACATATTGAACAGCAGCTTAAGCAATT
ACACGGTGTACACCAAGTCAACATAGAAATAGAAAGCAATAATGGCAAAATTTTCATTGATTTTGAAACCCCTGCAAGTT
TAAATAATTTAGAAAAAGAAGTTTATGATTTAGGATATGAAATTTTATATTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT154454 WP_001295795.1 NZ_CP051609:3963360-3964142 [Escherichia coli O25b:H4-ST131]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT154454 NZ_CP068063:387304-387597 [Staphylococcus equorum]
ATGGCGGAAATACAAAAAGCGCATCACTCAGAACAAATTAAATCTAATTTAAAATCAAGATTAAATCGTATAGAAGGCCA
AGTGAGAGCAATTAATCGCATGGTAGATGAAGATGTTTATTGTGATGACGTGCTCACACAAATTAGAGCCACTCGATCGG
CTTTAAATGGTGTTGCTACTAAATTATTAGACCATCATATGAAAAGTTGTATTATGGAAAAAGTAGAGCAGGGTCAACAA
GAAGAAGCTATGGAAGAACTATTGGTTACATTTCAAAAATTAATGAAGGATTAG

Structures

Toxin

• AlphaFold DB: A0A061KTE0