Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3685376..3685896 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag HHM37_RS17545 Protein ID WP_001160964.1
Coordinates 3685612..3685896 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HHM37_RS17540 Protein ID WP_000125566.1
Coordinates 3685376..3685609 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS17515 3680449..3680736 + 288 WP_000203744.1 ferredoxin-like protein FixX -
HHM37_RS17520 3680795..3682126 + 1332 WP_001183209.1 MFS transporter -
HHM37_RS17525 3682234..3682764 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HHM37_RS17530 3682757..3684619 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
HHM37_RS17535 3684811..3685290 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
HHM37_RS17540 3685376..3685609 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HHM37_RS17545 3685612..3685896 + 285 WP_001160964.1 CcdB family protein Toxin
HHM37_RS17550 3685923..3686771 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HHM37_RS17555 3686778..3687155 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HHM37_RS17560 3687158..3687979 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HHM37_RS17565 3687976..3688965 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HHM37_RS17570 3688965..3690251 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T154453 WP_001160964.1 NZ_CP051609:3685612-3685896 [Escherichia coli O25b:H4-ST131]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T154453 NZ_CP068061:c2142227-2141895 [Mammaliicoccus vitulinus]
ATGGTAAAACAGTACGATATTATTAAATTAAATCTAAATCCTGTTCAAGGCAATGAAAAAGGAAATTATAGACCATGTTT
AGTCGTAAATATTACAGAGTTCACGAAACAATCTGGTTTCGCATGGGTTATACCTATTACATCACGTAAACAGTCACGCT
ATCCTACAGATGTTATAGTTGAAACAGAAAAAGGATTGATTAATGGTGTTGTAGATTGTGCTCACATTAGAACAGTTGAT
TTATATACACGTTCTCATCAAAAAGTTGATGTACTAAAAGCAAATAAGATAGAAGAAGTAAAAGATATTCTAACAGGTAT
TATGGATTTGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT154453 WP_000125566.1 NZ_CP051609:3685376-3685609 [Escherichia coli O25b:H4-ST131]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT154453 NZ_CP068061:c2142481-2142221 [Mammaliicoccus vitulinus]
ATGAGTGAACCAACCAATAAGGCATATGCTAAAGTATATAAAAGTGGTAATGGCCAGGTTATCAGTATTAAAAAAGATAT
GTTAGAAAAAGCAGGTTTTAAGATAGGGGATGAACTTGTTATGAATGTTAAAGGTAATCAAATTGTATTAGAAAAACCTA
ATACATTCAAAGACCGTTGGAGAAAGTTTATTGAAGATGGTGGAGAATATGAACGTGGTGAATACGATTGGGGAGAATCA
GTGGGGCGTGAAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References