Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3575334..3575910 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag HHM37_RS17015 Protein ID WP_001295743.1
Coordinates 3575334..3575621 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag HHM37_RS17020 Protein ID WP_000063148.1
Coordinates 3575608..3575910 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS17000 3570994..3572298 - 1305 WP_000535012.1 restriction endonuclease subunit S -
HHM37_RS17005 3572288..3573907 - 1620 WP_001029745.1 type I restriction-modification system subunit M -
HHM37_RS17010 3573971..3575137 - 1167 WP_000800831.1 restriction endonuclease -
HHM37_RS17015 3575334..3575621 + 288 WP_001295743.1 BrnT family toxin Toxin
HHM37_RS17020 3575608..3575910 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
HHM37_RS17025 3575944..3576900 - 957 WP_001295745.1 GTPase -
HHM37_RS17030 3576911..3577114 - 204 WP_000467859.1 YbdD/YjiX family protein -
HHM37_RS17035 3577164..3579314 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T154452 WP_001295743.1 NZ_CP051609:3575334-3575621 [Escherichia coli O25b:H4-ST131]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T154452 NZ_CP068061:c1552220-1551849 [Mammaliicoccus vitulinus]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTCACTGGTTCTGAACAAGGGGGGACAAGACCAGTCGTAAT
TATCCAAAATGATACGGGTAACAGATATAGTCCGACTGTTATTGTAGCAGCAATTACTGGTAAGATAAATAAAGCTAAAA
TACCTACACACGTAGAAATTGAAGCAGCGAAATATAAACTAGATAGAGATTCAGTTATTTTGCTAGAACAGATTAGAACC
ATTGATAAGAAACGTTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATACAGCTATAGCAAT
CAGCCTCAACCTAACTTTGCAACAAAAATTTGATGCATTAGGTAATACATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT154452 WP_000063148.1 NZ_CP051609:3575608-3575910 [Escherichia coli O25b:H4-ST131]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT154452 NZ_CP068061:c1552388-1552221 [Mammaliicoccus vitulinus]
ATGACATCTTTAACACAAAATCGTACTCAAAGTATAGAACAAACACTGAAAGAAGGATATCGTTCTATGGCTGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATAGTGAATGTGAAGCTTGTGATTCTAATGAATCGCACTTAGCATCGCAACAAG
GTGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References