Detailed information of TA system TA154451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3525653..3526473
• Replicon: chromosome
• Accession: NZ_CP051609
• Organism: Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: HHM37_RS16780
• Protein ID: WP_001054379.1
• Coordinates: 3526216..3526473 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: HHM37_RS16775
• Protein ID: WP_000123957.1
• Coordinates: 3525653..3526204 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM37_RS16750	3521347..3522660	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
HHM37_RS16755	3522672..3522950	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
HHM37_RS16760	3522947..3524068	-	1122	WP_000010833.1	M42 family metallopeptidase	-
HHM37_RS16765	3524467..3524727	-	261	WP_000077645.1	hypothetical protein	-
HHM37_RS16770	3524855..3525601	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
HHM37_RS16775	3525653..3526204	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
HHM37_RS16780	3526216..3526473	-	258	WP_001054379.1	YjhX family toxin	Toxin
HHM37_RS16785	3526850..3527014	-	165	Protein_3277	GNAT family N-acetyltransferase	-
HHM37_RS16795	3528320..3529363	+	1044	WP_001363826.1	hypothetical protein	-
HHM37_RS16800	3529946..3530926	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T154451 WP_001054379.1 NZ_CP051609:c3526473-3526216 [Escherichia coli O25b:H4-ST131]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T154451 NZ_CP068061:c1379309-1379172 [Mammaliicoccus vitulinus]
TTGAATAAAAATCCTGAAGAAGAAAATGAAATGGTTCAAGACTTAGATGATGTAAAAGAGCTAGGTAAAGAAATGGAACA
AATTTCAGAACAGAACGACGAAGCGTATGAAGAAAAGAAAGAAGAACAAGACTCATAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT154451 WP_000123957.1 NZ_CP051609:c3526204-3525653 [Escherichia coli O25b:H4-ST131]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT154451 NZ_CP068061:c1379922-1379323 [Mammaliicoccus vitulinus]
ATGGCTTTAAATTTTAAAGTATTTGAAACAAAAGATATCGCTGCACGTTACGCTGCAGATGTAGTTCGTAAACAATTTAA
TTCAGACAGTACTTCAGTAGTTGGTATTCACTTAGATGCAGAAAACACTTCATTTTTTGATGAATTATTAGATGACGTAA
AGAAAAATCCAGTGAACTTTAGCCAAATTCATGTTTTAGATTTTGATGGTAAAGAAGATTACTACACAGAATTAGGTGTA
CCAAAAAAACAAGTCTTAAAAACTGATGTTGGTAGTGATGTAGAATCATTCATCAAAGATAAAGTGAACACTGATAAAAA
GAAAAATAAATTAATGTTAGATGTCATTACTTTAACTGAAGATAGTAGAACTGGATTAGATTATAATCAAGCGATACATC
CAGCTAGAGAAGTTATTGTACTTGTTACAGGTAAAGAAAAAGCTAAAGCAGTGAGTCAATTATATGAAGAGCCAGCAAAT
GGTTCAGTTGAATCAGCAAAATTAAAACAACATAGAATGGTCACAGTTTTATTAGATAACGAAGCGGCACAAGGATTGCC
AGAAGACGTTAGATCTTACTTCTCAATTCAATTTTCATAA

Structures

Toxin

• AlphaFold DB: B6I030