Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3493016..3493851 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag HHM37_RS16625 Protein ID WP_000854759.1
Coordinates 3493474..3493851 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag HHM37_RS16620 Protein ID WP_001295723.1
Coordinates 3493016..3493384 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS16595 3490131..3490289 + 159 WP_001323397.1 DUF905 family protein -
HHM37_RS16600 3490444..3491262 + 819 WP_001234738.1 DUF945 domain-containing protein -
HHM37_RS16605 3491604..3492077 + 474 WP_001350782.1 antirestriction protein -
HHM37_RS16610 3492093..3492569 + 477 WP_001186775.1 RadC family protein -
HHM37_RS16615 3492632..3492853 + 222 WP_000692345.1 DUF987 domain-containing protein -
HHM37_RS16620 3493016..3493384 + 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HHM37_RS16625 3493474..3493851 + 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
HHM37_RS16630 3493848..3494336 + 489 WP_000761690.1 hypothetical protein -
HHM37_RS16635 3494353..3494529 + 177 WP_000839286.1 DUF957 domain-containing protein -
HHM37_RS16640 3494629..3494787 + 159 WP_001467148.1 hypothetical protein -
HHM37_RS16645 3495151..3495327 + 177 Protein_3249 helix-turn-helix domain-containing protein -
HHM37_RS16650 3496100..3497740 + 1641 WP_001332039.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T154450 WP_000854759.1 NZ_CP051609:3493474-3493851 [Escherichia coli O25b:H4-ST131]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T154450 NZ_CP068060:c2176434-2176156 [Streptococcus anginosus]
ATGTATCAAGTCAAGTTTACCTCTGCTTTTAAAAAGAGCTATAAACGGATTAAAAAGCGAGGCTTAGAGATAGCACTCTT
AGATGAAGTGATTGAAAAACTCCGTCTAGGTCAGACACTAGAAGAAAAATACCGTGACCATGAACTCAAAGGAAACTTTG
TTGGCTTTAGGGAATGTCACATCAAGCCAGATTGGCTCTTGATTTATCTGATTGAAGACGATATTTTGACATTGACTTTA
GCTGATACAGGCAGTCATGCGGATTTGTTTAAGATGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT154450 WP_001295723.1 NZ_CP051609:3493016-3493384 [Escherichia coli O25b:H4-ST131]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT154450 NZ_CP068060:c2176707-2176435 [Streptococcus anginosus]
ATGGCAACTGTACCAACTCAAATTCGGATTAATGAAACTGTCAAAGCCCAAGCAACTTCATTATTTAATGACTTGGGGAT
GGATATGTCTAGTGCAGTCAATATTTTTCTTCGGCAATGTCTATTGCGTGGTGGACTTCCTTTCACGGTTGAAGTTCCCA
ATTATAGCCAAAAGACCTTAGAAGCTCTGGCAGAAGCTAAACGAATCTCTCGTGATGAAACGGTCGCTTCTTATGACAAT
CTAGCAGATTTAAAGAAGGCTCTGGAAGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References