Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3396203..3396798 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag HHM37_RS16145 Protein ID WP_000239579.1
Coordinates 3396448..3396798 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag HHM37_RS16140 Protein ID WP_001223208.1
Coordinates 3396203..3396454 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS16130 3391868..3395647 + 3780 WP_000060945.1 autotransporter assembly complex protein TamB -
HHM37_RS16135 3395650..3395991 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
HHM37_RS16140 3396203..3396454 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
HHM37_RS16145 3396448..3396798 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
HHM37_RS16150 3396878..3397408 - 531 WP_000055075.1 inorganic diphosphatase -
HHM37_RS16155 3397718..3398674 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
HHM37_RS16160 3398814..3400316 + 1503 WP_000205813.1 sugar ABC transporter ATP-binding protein -
HHM37_RS16165 3400330..3401352 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T154449 WP_000239579.1 NZ_CP051609:3396448-3396798 [Escherichia coli O25b:H4-ST131]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T154449 NZ_CP068060:c2172517-2172263 [Streptococcus anginosus]
ATGTTTAACTTTACAGAACAAGCTTGGAAAGATTATGTTTCTTGGCAACAAGAAGATAAAAAGATACTCAAACGTATCAA
TCGTTTAATGGAGGATATCAAACGAAATCCTTTTGAAGGGATTGGAAAGCCTGAACCCTTAAAATACCGTTATTCCGGTG
CTTGGTCTCGTAGAATTACAGATGAACACCGATTAGTCTATATGGTGGAAGAAAATGAGATTTATTTCTTATCCTTTCGA
GACCACTATAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT154449 WP_001223208.1 NZ_CP051609:3396203-3396454 [Escherichia coli O25b:H4-ST131]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT154449 NZ_CP068060:c2172776-2172510 [Streptococcus anginosus]
ATGGAAGCTGTCGTCTATTCAAATTTTAGAAACAATTTAAAGAATTACATGAAAAAAGTCAACGATGAATATGAACCTCT
AATGGTTGTCAATAAAAATCCTGATGAAAATATTGTGGTCTTATCTAAGGAAAACTGGGATAGTATTCAAGAAACCATTC
GCCTAATGAATAATGATTATCTTTCAGACAAGGTTCTATCAGGAATGGAACAAGTCAAGCAAAAGAAAGTCATTCAACGC
CCATTATTAGAGGTGGAAGATGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References