Detailed information of TA system TA154448

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3254040..3254841
• Replicon: chromosome
• Accession: NZ_CP051609
• Organism: Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D8EBK0
• Locus tag: HHM37_RS15390
• Protein ID: WP_001094436.1
• Coordinates: 3254040..3254417 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MN76
• Locus tag: HHM37_RS15395
• Protein ID: WP_015953067.1
• Coordinates: 3254464..3254841 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHM37_RS15360	3249999..3250160	-	162	Protein_2997	DUF4942 domain-containing protein	-
HHM37_RS15365	3250251..3251750	+	1500	WP_029700729.1	IS21-like element ISEc10 family transposase	-
HHM37_RS15370	3251747..3252502	+	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
HHM37_RS15375	3252566..3253261	-	696	Protein_3000	DUF4942 domain-containing protein	-
HHM37_RS15380	3253346..3253543	-	198	WP_000839254.1	DUF957 domain-containing protein	-
HHM37_RS15385	3253555..3254043	-	489	WP_000761714.1	hypothetical protein	-
HHM37_RS15390	3254040..3254417	-	378	WP_001094436.1	TA system toxin CbtA family protein	Toxin
HHM37_RS15395	3254464..3254841	-	378	WP_015953067.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HHM37_RS15400	3254920..3255141	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HHM37_RS15405	3255210..3255686	-	477	WP_001186756.1	RadC family protein	-
HHM37_RS15410	3255701..3256186	-	486	WP_000860054.1	antirestriction protein	-
HHM37_RS15415	3256277..3257095	-	819	WP_048266340.1	DUF945 domain-containing protein	-
HHM37_RS15420	3257185..3257418	-	234	WP_001278287.1	DUF905 family protein	-
HHM37_RS15425	3257424..3258101	-	678	WP_001097301.1	hypothetical protein	-
HHM37_RS15430	3258249..3258929	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3247083..3256186	9103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13958.84 Da
• Isoelectric Point: 6.8603

>T154448 WP_001094436.1 NZ_CP051609:c3254417-3254040 [Escherichia coli O25b:H4-ST131]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T154448 NZ_CP068060:1937167-1937358 [Streptococcus anginosus]
ATGCCACTTACAGGAAAAGAAATGGCAAAGCTAGCCGAGAAAAATGGTTGGGTAGAAATCCGACAGAACGGCAGCCATCA
CCATTTCAAACATGAAGATTTTGATTATATAGTGACTATCCCAATCCATGGAAACAAGGATTTAGGAAGAGGGCTAGAAA
GCAAAATCCTGAAAGATTTGGGGCTGAAATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13766.60 Da
• Isoelectric Point: 6.2021

>AT154448 WP_015953067.1 NZ_CP051609:c3254841-3254464 [Escherichia coli O25b:H4-ST131]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 378 bp

>AT154448 NZ_CP068060:1937394-1937774 [Streptococcus anginosus]
ATGTTAAAATCATATCCAGCAATTTTTCATAAAGAGGAAGACGGTTCTTATTGGGTAGAATTTCCAGAGTTTGGAGGAGG
CACACAAGGGGCAAATGTTGAAGAAGCCTTGAAAAATGCCCGTGAAATGCTTGAGAGTGTTTTAGCTGCTTACATAGACG
AAGGATTAGATATGCCAAAGCCTAGCGACATCATGGCTTTAACCGTTAAAGACGGCTTTGTTAGCATGATACAAGCAGAC
CCTACCCCGTTTATTCGCAACAATAAAGCCGTTAGAAAGAATGTGACTGTCCCTGAATGGCTAGTAAGATTAGCGGATCG
TGAAAAAGTAAATTATTCTGAGGTGCTAACTCAAGCCCTAGAAACAAAGTTACAAGCCTAG

Structures

Toxin

• AlphaFold DB: A0A0E2L1N0

Antitoxin

• AlphaFold DB: A0A5Z3CIS0