Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3238239..3238736 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HHM37_RS15325 Protein ID WP_001173343.1
Coordinates 3238563..3238736 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HHM37_RS15320 Protein ID WP_001312331.1
Coordinates 3238239..3238535 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS15310 3233596..3237525 + 3930 WP_001080129.1 autotransporter outer membrane beta-barrel domain-containing protein -
HHM37_RS15315 3237739..3238011 + 273 WP_000405647.1 N-acetyltransferase -
HHM37_RS15320 3238239..3238535 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HHM37_RS15325 3238563..3238736 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HHM37_RS15330 3238855..3240372 - 1518 WP_000003806.1 lysine--tRNA ligase -
HHM37_RS15335 3240609..3242066 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154447 WP_001173343.1 NZ_CP051609:3238563-3238736 [Escherichia coli O25b:H4-ST131]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154447 NZ_CP068060:c1051044-1050256 [Streptococcus anginosus]
ATGAGACTGGAAGAATTTAGTGAGGATGAATTTCAGAAAGCCTTACAGCGAACGATTCGATCTCTAACTCTAACTCGTGG
AAAGACGATTCCAGACCAACCGAAAGCCATTTTGCTTGGAGGTCAAAGCGGAGCTGGTAAGACGACTATTCATCGAATTA
AGCAAAAAGAATTTCAAGGGAATATCATTATCATTGACGGTGATAGCTACCGTTCTCAGCACCCAAATTACTTAGCTCTG
CAAGAAAAGTATGGTAAAGATAGTGTGGATTATACAAAGGGATTTGCAGGAAAAATGGTAGAGCATCTGGTTGATGAACT
CAGTACACAAGGCTATCATTTGTTGATTGAAGGGACTTTGCGAACCACTCAAGTCCCACGGAAGACTGCTCAATTACTAC
GAAAAAAAGGCTACCATATTTCTTTGGCTGTGATTGGCACCAAACCAGAACTTTCCTATCTCAGTACCTTGATTCGTTAT
GAGGAACTTTACACAATAAATCCTAATCAGGCCAGAGCAACCCCTAAAGAACATCATGATGGTATTGTAGAGCATTTGGT
TGATAACCTAAGAGAGTTAGAAAACGATAAACTCTTTGACCGCATTCAAATTTACCAAAGAGATAGGACTTGTATTTACG
ATTCTGAAACGGATGAGATTTCCGCAGCAACAGTTCTTCAAGAATGCCTTTTCGGAAAATGGAGTAAGGTTGAGGAGGAG
ATGTTGAAAGTGGGACAGGAACGGTTGAGAGATTTGTGTTGGAAGAACGGTAGTAGTGGTATCTTCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT154447 WP_001312331.1 NZ_CP051609:3238239-3238535 [Escherichia coli O25b:H4-ST131]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154447 NZ_CP068060:c1051520-1051044 [Streptococcus anginosus]
ATGATTGGAGATAATATTAAGTCCTTACGAAAAACTCATGATTTAACCCAGCCAGAATTCGCAAAAATTGTTGGGATTTC
ACGCAATAGCCTAAGTCGATATGAAAATGGAACTAGTTCTGTTTCAACAGAACTCATCGACCGAATTTGTCAGAAATTTA
ATGTTTCATACGTTGATATTGTAGGGGAGGACAAAATGCTAACACCCCTTGAAGATTATCAATTGACCTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCGGCCATTTTATCCCAACTCTATCGCTACCAAGATAGTCAAGATATTGCTTTTGATGACGA
AACCAATCCTTGGATTTTGATGAGTGATGATTTGGCTGAATTGATCCATACAAAGATTTACCTTGTTGATACTTTCGATG
AAATAGAGCGTTATAATGGCTATTTAGACGGTATAGAGCGGATGTTAGACACGGTGTATCATCGGGTGGTGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References