Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2388229..2389029 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag HHM37_RS11490 Protein ID WP_000342452.1
Coordinates 2388229..2388756 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag HHM37_RS11495 Protein ID WP_001277107.1
Coordinates 2388763..2389029 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS11465 2383305..2384072 - 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HHM37_RS11470 2384069..2385346 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
HHM37_RS11475 2385343..2386269 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HHM37_RS11480 2386317..2387426 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
HHM37_RS11485 2387849..2388232 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
HHM37_RS11490 2388229..2388756 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
HHM37_RS11495 2388763..2389029 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
HHM37_RS11500 2389178..2390281 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
HHM37_RS11505 2390552..2391406 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
HHM37_RS11510 2391651..2392709 - 1059 WP_001042013.1 permease-like cell division protein FtsX -
HHM37_RS11515 2392702..2393370 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T154445 WP_000342452.1 NZ_CP051609:c2388756-2388229 [Escherichia coli O25b:H4-ST131]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T154445 NZ_CP068057:1354555-1354902 [Streptococcus dysgalactiae]
TTGGTATCTGATAATAAAACCCATAGCCTTATTATCCCCGAAACCGTACAAGAACAATTACAGGAGATAAAAAGCTATGT
TGAAACTACTTACTTTTCGGAGCAGGCAGGGGCTAACACCGTCAACAATATTCTTTACGGGCTAGAACGTCTTGAATTTT
TCCCAGAAGCAGGATTTAACGCAGATGACAGAGTAGGAGAAACTATTTACCCGCCACATAATACCCGTTGCATTGTTTTA
GGGGACTACCTAGCTTTTTATCACATTTTAGAAGACAGAAAAGTCGTTTTTGTATCTGACATTATCCATAGCAAACAAGA
CTATATCAGACTGTTTAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT154445 WP_001277107.1 NZ_CP051609:c2389029-2388763 [Escherichia coli O25b:H4-ST131]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT154445 NZ_CP068057:1354278-1354565 [Streptococcus dysgalactiae]
ATGCACGTTTTAGAAAAAAATACACAAGTCAATTTTAAAACCAATAGCGAATTATTAGAGAAAGCTAAAGCTATTATCGC
AGCCCAAAACCTTGACATGACAGCTAGCTTTAATTTGTTTTTAGAAAACATTGTACAAAATAAAGCCTTACCTTTTGAGA
CTGACACCGATAGAGAACGAACTGAACTACTTGCAGGTTTACGTGCTGAAATTGCTAAAAGTTTTGACGATTTAGAGCAT
GGGCGGGTATACAACGCTAGTGAAGTGAGGGCTAACCTTGGTATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References