Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 777264..778095 Replicon chromosome
Accession NZ_CP051609
Organism Escherichia coli O25b:H4-ST131 strain 2019_APHA

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag HHM37_RS03895 Protein ID WP_000854815.1
Coordinates 777721..778095 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag HHM37_RS03890 Protein ID WP_001280918.1
Coordinates 777264..777632 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHM37_RS03850 772350..773096 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
HHM37_RS03855 773179..773529 + 351 Protein_759 hypothetical protein -
HHM37_RS03860 773545..773955 + 411 WP_000846703.1 hypothetical protein -
HHM37_RS03865 774176..774994 + 819 WP_001542275.1 DUF945 domain-containing protein -
HHM37_RS03870 775336..775809 + 474 WP_001542276.1 antirestriction protein -
HHM37_RS03875 775825..776301 + 477 WP_001186200.1 RadC family protein -
HHM37_RS03880 776364..776585 + 222 WP_000692345.1 DUF987 domain-containing protein -
HHM37_RS03885 776604..777248 + 645 WP_000086752.1 hypothetical protein -
HHM37_RS03890 777264..777632 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HHM37_RS03895 777721..778095 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
HHM37_RS03900 778092..778286 + 195 WP_000988600.1 hypothetical protein -
HHM37_RS03905 778299..778412 + 114 WP_001161660.1 DUF957 domain-containing protein -
HHM37_RS03910 778701..778847 - 147 Protein_770 transposase domain-containing protein -
HHM37_RS03915 778901..779083 + 183 WP_001296208.1 hypothetical protein -
HHM37_RS03920 779184..779513 - 330 WP_000450409.1 DUF496 family protein -
HHM37_RS03925 779685..780743 - 1059 WP_001200889.1 FUSC family protein -
HHM37_RS03930 780941..781414 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
HHM37_RS03935 781533..782699 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T154439 WP_000854815.1 NZ_CP051609:777721-778095 [Escherichia coli O25b:H4-ST131]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T154439 NZ_CP068050:3760880-3761179 [Burkholderia gladioli]
ATGTGGGCTGAGGCACGGGCGGTGCGACTGACGCCGTTGGCCGAGGCGGATCTCGAGGATATCTGGACCTACACGTTCGA
GCAGTGGTCACTCGAGCAGGCCGAGCGCTACGTGGGCGACCTGATTGAGGCATTCGAGCGCCTGGCACGCGGCGATTGGG
TAGGGCGATCTAGCCGCGCGAGCGATGACTATCGGCGTTACCTGGTGGGGTCTCACGTGGTCTTCTATCGGGAAACGAAG
GCTACCCTAGACGTGATTCGCGTGCTGCATCAGCGCATGGACGTCGACCGGCATTTGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT154439 WP_001280918.1 NZ_CP051609:777264-777632 [Escherichia coli O25b:H4-ST131]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT154439 NZ_CP068050:3760645-3760890 [Burkholderia gladioli]
ATGCCGCGGAATACATCGATCTCGCTGGGGGATCACCTGACCCAGTTCGTGGATACCCAGGTCGCGTCCGGGCGCTATGG
CTCGGCCAGCGACGTGGTACGGGCCGGTTTGCGGCTGCTCGAGACGCAGGAAACCGAGCTAAAGGCCTTGCAGGAAGCAC
TGCGCGCAGGTGAGGCATCCGGCGCGCCGGCGGCCTTCGACGGCAAGGCGTTCCTTGCGCGCATGCGCGTCAAGCATGTG
GGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References