Detailed information of TA system TA154429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 29034..29677
• Replicon: plasmid unnamed1
• Accession: NZ_CP051549
• Organism: Phytobacter diazotrophicus strain UAEU22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HHA33_RS25775
• Protein ID: WP_169040146.1
• Coordinates: 29261..29677 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HHA33_RS25770
• Protein ID: WP_085007143.1
• Coordinates: 29034..29264 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHA33_RS25740	24333..25262	+	930	WP_004118344.1	copper resistance inner membrane protein PcoD	-
HHA33_RS25745	25317..25997	+	681	WP_001188930.1	copper response regulator transcription factor PcoR	-
HHA33_RS25750	25994..27394	+	1401	WP_007374420.1	copper resistance membrane spanning protein PcoS	-
HHA33_RS25755	27612..28046	+	435	WP_007374421.1	hypothetical protein	-
HHA33_RS25760	28424..28522	-	99	WP_004118348.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HHA33_RS25765	28509..28688	-	180	WP_004118349.1	type II toxin-antitoxin system ParD family antitoxin	-
HHA33_RS25770	29034..29264	+	231	WP_085007143.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HHA33_RS25775	29261..29677	+	417	WP_169040146.1	type II toxin-antitoxin system VapC family toxin	Toxin
HHA33_RS25780	29864..31093	-	1230	WP_047368853.1	HDOD domain-containing protein	-
HHA33_RS25785	32660..32902	-	243	WP_047368854.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / aadA2	-	1..104972	104972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15068.40 Da
• Isoelectric Point: 7.2449

>T154429 WP_169040146.1 NZ_CP051549:29261-29677 [Phytobacter diazotrophicus]
VNKTYMLDTNICLLIMREQPEAVLKRLEQAVLRNHRTVISAITYQEMRSGATGPKASPRHIALVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAVAAGAILVTNNTREFERVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T154429 NZ_CP068049:c3080784-3080200 [Burkholderia gladioli]
ATGGACGACGCGCTGATCGACGCACGACTGGATTTCCTGCGCGGCTGCGAGCGCCTGAAGGATGTGCTGCGCAGCGGCCA
TACCTCGGGTGGCCGACGCGAGAGCACGGCCGAGCACAGCTGGCGGCTCGCGCTGATGGCGATGGTGCTGATGGATCAAT
TGGGCGAGGTCGACCCCGGCAGGGTGCTCGAGCTCTGCCTGGTGCATGACCTGGGCGAGGCGCTGGGCGGCGACGTGCCG
GCGCCCGAGCAGGCCCCGGGCAGCGACAAGGATGCGGTCGAGCGACGCGATCTGCTGCGCCTCGGCGAAACGCTCGATGC
GCCGATGCGCGAGAGGATCGTCGCGTTGTGGGACGAGTATGCGCGGGCGGCCACGCCGGAAGCACGCGCGGTGAAGGCGC
TCGACAAGATCGAGACGATCCTGCAGCACACGCAGGGCGAGAATCCGCCCGATTTCGACTATGCGTTCAATCTCTCGTAT
GGGCAGGCGCATACGCGCAAGGTGCCGGCGCTGCAACGGTTTCGCGAGCGCGTCGACGAGGCGACGCGCGAGCGGATGAC
CCGTGCTGGCGGGCCTTCGGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8750.95 Da
• Isoelectric Point: 4.6583

>AT154429 WP_085007143.1 NZ_CP051549:29034-29264 [Phytobacter diazotrophicus]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVREGDRIILRPVRPAWGSFAKMEKADPDFMAEREDVVSDEGRVDL

Nucleotide

• Length: 231 bp

>AT154429 NZ_CP068049:c3081438-3080887 [Burkholderia gladioli]
TTGCCGCCGTCCGCGCTGGCCTGTTCGCCGGGCGCGAGCTTGCGTCGATGGCGCACGCTGCGCCGGATCAAGCAGGAACA
TGCGGCGGCGCTGTTCGGCGTATCGCAGTCGACGATATCGCGCTGGGAAGCCGGCACGCAGGCGGTGTCGCACGAGGAAC
TGCGGCGCATCGAGGCCTGCTTGTCGGCGCGCCTCGACTCGGCGGCGGATCGCGCGCTCGAGCGCCTCGTCAGCCATCAC
ACCCGTCCCGTTCATCTCGTCTGCGACCTCACGCACCGGCTGCTGGCCTGCTCCACGCCGCGAGCCGCGGAGTTCGCGGC
GCCGCTGTCGTCGCTGATGGGCGCGTCGCTCTGGCCGTTCGCGACGGCCGAGATCGTGGCGCGCGAGCAGTCGTTGCGCG
ATGCGGGCTGGCACGATCATCCGGCGCCCTCGATCGAATTCGATACCGGGTCCAACGATTCGCCTCAGGTGCCGATTCGT
GCGAGCCGCTGCCGCTGGACGCGTTTCACCTTGTCCGACGGGACGCTGGCGCGCCTGGTCGAAACGCTTTGA