Detailed information of TA system TA154419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3196338..3196981
• Replicon: chromosome
• Accession: NZ_CP051548
• Organism: Phytobacter diazotrophicus strain UAEU22

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HHA33_RS15360
• Protein ID: WP_169039363.1
• Coordinates: 3196338..3196754 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A348DE82
• Locus tag: HHA33_RS15365
• Protein ID: WP_071194209.1
• Coordinates: 3196751..3196981 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHA33_RS15340	3192587..3193348	-	762	WP_169039360.1	ABC transporter ATP-binding protein	-
HHA33_RS15345	3193348..3194385	-	1038	WP_169039361.1	iron ABC transporter permease	-
HHA33_RS15350	3194385..3195383	-	999	WP_169039362.1	ABC transporter substrate-binding protein	-
HHA33_RS15355	3195668..3196075	+	408	WP_169040090.1	nuclear transport factor 2 family protein	-
HHA33_RS15360	3196338..3196754	-	417	WP_169039363.1	PIN domain-containing protein	Toxin
HHA33_RS15365	3196751..3196981	-	231	WP_071194209.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HHA33_RS15370	3197239..3198525	+	1287	WP_169039364.1	MFS transporter	-
HHA33_RS15375	3198601..3199365	-	765	WP_039079009.1	SDR family oxidoreductase	-
HHA33_RS15380	3199417..3200370	-	954	WP_169039365.1	2-hydroxyacid dehydrogenase	-
HHA33_RS15385	3200441..3200818	-	378	WP_108701294.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15098.61 Da
• Isoelectric Point: 9.2345

>T154419 WP_169039363.1 NZ_CP051548:c3196754-3196338 [Phytobacter diazotrophicus]
MKKTFMLDTNICSFIMREHPESVIKRLEQAVLRNNRIVVSAITYAEMRFGAIGKKASPHHAKLVDAFCARLDAVLAWDRA
AVDATTQIKLALALAGTPIGPNDTAIAGHAIAAGFVLVTNNTREFERVPGLILEDWAK

Nucleotide

• Length: 417 bp

>T154419 NZ_CP068041:c3980327-3980031 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8783.91 Da
• Isoelectric Point: 4.3654

>AT154419 WP_071194209.1 NZ_CP051548:c3196981-3196751 [Phytobacter diazotrophicus]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDCIILRPVRPTWSSFAQLEKADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT154419 NZ_CP068041:c3980029-3979634 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A348DE82