Detailed information of TA system TA154416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2410813..2411403
• Replicon: chromosome
• Accession: NZ_CP051548
• Organism: Phytobacter diazotrophicus strain UAEU22

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HHA33_RS11455
• Protein ID: WP_169039137.1
• Coordinates: 2411071..2411403 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A7M2V158
• Locus tag: HHA33_RS11450
• Protein ID: WP_041851870.1
• Coordinates: 2410813..2411070 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHA33_RS11425	2406418..2406753	-	336	WP_132439155.1	gamma-glutamylcyclotransferase	-
HHA33_RS11430	2406881..2407171	-	291	WP_169039135.1	DUF3601 domain-containing protein	-
HHA33_RS11435	2407429..2407968	-	540	WP_079496546.1	ester cyclase	-
HHA33_RS11440	2408268..2409377	-	1110	WP_169039136.1	alkene reductase	-
HHA33_RS11445	2409478..2410389	+	912	WP_125124237.1	LysR family transcriptional regulator	-
HHA33_RS11450	2410813..2411070	+	258	WP_041851870.1	antitoxin	Antitoxin
HHA33_RS11455	2411071..2411403	+	333	WP_169039137.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HHA33_RS11465	2411808..2413241	+	1434	WP_107224097.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HHA33_RS11475	2413495..2414406	-	912	WP_107224098.1	sugar ABC transporter permease	-
HHA33_RS11480	2414425..2415699	-	1275	WP_071196351.1	extracellular solute-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11699.39 Da
• Isoelectric Point: 8.0290

>T154416 WP_169039137.1 NZ_CP051548:2411071-2411403 [Phytobacter diazotrophicus]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSPASFNALTRLSVVVPVTSGGNFARAAGFAVSLDGAGTKTTGVIRCDQPRTI
DMDARNGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T154416 NZ_CP068041:c2976337-2976086 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9286.76 Da
• Isoelectric Point: 4.5799

>AT154416 WP_041851870.1 NZ_CP051548:2410813-2411070 [Phytobacter diazotrophicus]
MYIARLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIENGCLIIEPQKRPHYSLEELLAQCDPHAEISEEDRQWIDAPAA
GKELL

Nucleotide

• Length: 258 bp

>AT154416 NZ_CP068041:c2976089-2975835 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7M2V158