Detailed information of TA system TA154414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1323432..1324213
• Replicon: chromosome
• Accession: NZ_CP051548
• Organism: Phytobacter diazotrophicus strain UAEU22

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HHA33_RS06310
• Protein ID: WP_125123871.1
• Coordinates: 1323722..1324213 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HHA33_RS06305
• Protein ID: WP_125123870.1
• Coordinates: 1323432..1323725 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHA33_RS06295	1320429..1320593	+	165	WP_110295761.1	DUF1778 domain-containing protein	-
HHA33_RS06300	1320643..1322832	-	2190	WP_169038827.1	TonB-dependent siderophore receptor	-
HHA33_RS06305	1323432..1323725	+	294	WP_125123870.1	DUF1778 domain-containing protein	Antitoxin
HHA33_RS06310	1323722..1324213	+	492	WP_125123871.1	GNAT family N-acetyltransferase	Toxin
HHA33_RS06315	1324278..1324976	+	699	WP_169038828.1	hypothetical protein	-
HHA33_RS06320	1324963..1325478	-	516	WP_125123872.1	hypothetical protein	-
HHA33_RS06325	1325633..1326784	+	1152	Protein_1221	IS3 family transposase	-
HHA33_RS06335	1327258..1327977	-	720	WP_039078045.1	DUF554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17721.58 Da
• Isoelectric Point: 7.2655

>T154414 WP_125123871.1 NZ_CP051548:1323722-1324213 [Phytobacter diazotrophicus]
MISAPEPLHTGHILTPFCCGMDSMDKWLKQRAMKNQVTGASRTFVCCGSDSNVQAYYSLASSAVTTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
ESI

Nucleotide

• Length: 492 bp

>T154414 NZ_CP068041:2964193-2964567 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10931.55 Da
• Isoelectric Point: 8.6351

>AT154414 WP_125123870.1 NZ_CP051548:1323432-1323725 [Phytobacter diazotrophicus]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYEEFLARLDQAPAPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT154414 NZ_CP068041:2963736-2964104 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA