Detailed information of TA system TA154386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 64509..65089
• Replicon: plasmid pSBP2_mcr4
• Accession: NZ_CP051531
• Organism: Shewanella baltica strain 11FHM2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A9L6F9
• Locus tag: HHE93_RS23145
• Protein ID: WP_006087375.1
• Coordinates: 64509..64754 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HHE93_RS23150
• Protein ID: WP_006087376.1
• Coordinates: 64754..65089 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHE93_RS23120 (HHE93_23215)	61418..61873	+	456	WP_014615256.1	type II toxin-antitoxin system HicB family antitoxin	-
HHE93_RS23125 (HHE93_23220)	61875..62363	+	489	WP_220774012.1	hypothetical protein	-
HHE93_RS23130 (HHE93_23225)	62394..62615	+	222	WP_014615254.1	hypothetical protein	-
HHE93_RS23135 (HHE93_23230)	63286..63909	+	624	WP_220774014.1	hypothetical protein	-
HHE93_RS23140 (HHE93_23235)	63926..64312	+	387	WP_259216204.1	hypothetical protein	-
HHE93_RS23145 (HHE93_23240)	64509..64754	+	246	WP_006087375.1	type II toxin-antitoxin system HicA family toxin	Toxin
HHE93_RS23150 (HHE93_23245)	64754..65089	+	336	WP_006087376.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HHE93_RS23155 (HHE93_23250)	65693..66694	+	1002	WP_259216206.1	zincin-like metallopeptidase domain-containing protein	-
HHE93_RS23160 (HHE93_23255)	66735..67046	+	312	WP_259216207.1	hypothetical protein	-
HHE93_RS23165 (HHE93_23260)	67400..68530	+	1131	WP_014615249.1	site-specific integrase	-
HHE93_RS23170 (HHE93_23265)	68632..69882	+	1251	WP_259216249.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-4.3	-	1..86727	86727

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (19-71)

Antitoxin

• Uncharacterised protein family HicB (55-104)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9200.75 Da
• Isoelectric Point: 10.5259

>T154386 WP_006087375.1 NZ_CP051531:64509-64754 [Shewanella baltica]
MTKTDKLLAKLKSSKTLTWNELAGALKVLGYQQVEGDGSRVKFDNGIPKELINLHKPHPKNELKAYALRQVRDKLTEWGK
L

Nucleotide

• Length: 246 bp

>T154386 NZ_CP068035:c3667285-3666887 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12145.72 Da
• Isoelectric Point: 5.0494

>AT154386 WP_006087376.1 NZ_CP051531:64754-65089 [Shewanella baltica]
MQNTMTYKGYHGSVEISPEDNILFGQVLFISPLINYEAETAKGLEQAFQEAINAYLADCAQQDIQPEKPCKGSLNVRLGH
DLHLAASIAAFQASTSTNEFIKRAVQKSVGL

Nucleotide

• Length: 336 bp

>AT154386 NZ_CP068035:c3667512-3667285 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• AlphaFold DB: A9L6F9