Detailed information of TA system TA154376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 737473..738038
• Replicon: chromosome
• Accession: NZ_CP051522
• Organism: Treponema denticola strain OMZ 910

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M2BU34
• Locus tag: E4N87_RS03365
• Protein ID: WP_010694033.1
• Coordinates: 737473..737811 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E4N87_RS03370
• Protein ID: WP_253699002.1
• Coordinates: 737805..738038 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N87_RS03340 (E4N87_03330)	733066..733485	-	420	WP_253699008.1	Rrf2 family transcriptional regulator	-
E4N87_RS03345 (E4N87_03335)	733494..734363	-	870	WP_253699007.1	Sir2 silent information regulator family NAD-dependent deacetylase	-
E4N87_RS03350 (E4N87_03340)	734381..735196	-	816	WP_253699006.1	protein-ADP-ribose hydrolase	-
E4N87_RS03355 (E4N87_03345)	735324..736367	-	1044	WP_253699005.1	DMT family transporter	-
E4N87_RS03360 (E4N87_03350)	736528..737370	-	843	WP_253717714.1	hypothetical protein	-
E4N87_RS03365 (E4N87_03355)	737473..737811	-	339	WP_010694033.1	endoribonuclease MazF	Toxin
E4N87_RS03370 (E4N87_03360)	737805..738038	-	234	WP_253699002.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4N87_RS03375 (E4N87_03365)	738513..738890	-	378	WP_253717716.1	hypothetical protein	-
E4N87_RS03380 (E4N87_03370)	739003..739179	-	177	WP_253717718.1	hypothetical protein	-
E4N87_RS03385 (E4N87_03375)	739229..739456	-	228	WP_253699000.1	PIN domain-containing protein	-
E4N87_RS03390 (E4N87_03380)	739453..739680	-	228	WP_253698999.1	DUF2281 domain-containing protein	-
E4N87_RS03395 (E4N87_03385)	739764..742139	-	2376	WP_253717722.1	endopeptidase La	-
E4N87_RS03400 (E4N87_03390)	742184..742345	+	162	WP_253718311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12821.66 Da
• Isoelectric Point: 6.6952

>T154376 WP_010694033.1 NZ_CP051522:c737811-737473 [Treponema denticola]
MVNSNYVPDRGDLVWLDFNPQAGHEQKGRRPAICISQKRYNQKVGLALFCPITSHIKGYPFEIVLDNHSINGCILSDQVK
NLDYKKRSCDFIEKTTEEEINAVVDNIKLLIE

Nucleotide

• Length: 339 bp

>T154376 NZ_CP068035:c1463139-1462921 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8624.85 Da
• Isoelectric Point: 5.6423

>AT154376 WP_253699002.1 NZ_CP051522:c738038-737805 [Treponema denticola]
MQVVVQKWGNSLGFRIPSLWAKDNNVKHGSKIEVIAEKGKIVILPQEKNLDDMLAMVTSENVHSEISTGYSVGNEEW

Nucleotide

• Length: 234 bp

>AT154376 NZ_CP068035:c1463539-1463165 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: M2BU34