Detailed information of TA system TA154365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 55087..56862
• Replicon: chromosome
• Accession: NZ_CP051515
• Organism: Paraburkholderia aromaticivorans strain AR20-38

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HF916_RS12035
• Protein ID: WP_168789185.1
• Coordinates: 55087..55977 (+)
• Length: 297 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HF916_RS12040
• Protein ID: WP_206001871.1
• Coordinates: 55978..56862 (+)
• Length: 295 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF916_RS12005	50442..51515	-	1074	WP_168789179.1	porin	-
HF916_RS12010	51957..52271	+	315	WP_168789180.1	non-heme iron oxygenase ferredoxin subunit	-
HF916_RS12015	52273..52584	+	312	WP_168789181.1	hypothetical protein	-
HF916_RS12020	52633..53727	+	1095	WP_168789182.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
HF916_RS12025	53720..54487	+	768	WP_168789183.1	SDR family oxidoreductase	-
HF916_RS12030	54514..55041	+	528	WP_168789184.1	cupin domain-containing protein	-
HF916_RS12035	55087..55977	+	891	WP_168789185.1	alpha/beta fold hydrolase	Toxin
HF916_RS12040	55978..56862	+	885	WP_206001871.1	helix-turn-helix domain-containing protein	Antitoxin
HF916_RS12045	56862..57659	+	798	WP_168789186.1	SDR family oxidoreductase	-
HF916_RS12050	57652..59310	+	1659	WP_168789187.1	thiamine pyrophosphate-binding protein	-
HF916_RS12055	59303..60133	+	831	WP_168789188.1	aspartate dehydrogenase	-
HF916_RS12060	60173..61663	+	1491	WP_168791973.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (50-279)

Antitoxin

• Transcription regulator IclR, C-terminal (148-272)

Sequences

Toxin

Protein

• Length: 297 a.a.
• Molecular weight: 30987.03 Da
• Isoelectric Point: 7.1058

>T154365 WP_168789185.1 NZ_CP051515:55087-55977 [Paraburkholderia aromaticivorans]
MSAVPSVTADQHATLEARLARFPAQQIGLASQRAVSYREVDSADSSALPLVLLHGIGSGAASWVQQFEGLGATRRVLAWD
APGYGASTPVAAESPAAADYATVLKEWLDALGIERCVLLGHSLGAVVAGAFAVTHPQRVAGLLLLSPAGGYGAASADVRN
TKRDQRLAMLNELGPQGLAEKRSTNMLSAHASDEARAWVRWNMSRVIPLGYAQATHLLANADLAGDLARFKGRINVAVGA
DDTITTPEACERLARAAGTQLQVVPRAGHAGYIEAPAAYTAIIDTFCRTSDGPRSQ

Nucleotide

• Length: 891 bp

>T154365 NZ_CP068033:3933402-3933620 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 295 a.a.
• Molecular weight: 32608.17 Da
• Isoelectric Point: 6.8844

>AT154365 WP_206001871.1 NZ_CP051515:55978-56862 [Paraburkholderia aromaticivorans]
MTPDTISAERDADEDRNGTTGDTAYRVPGLERGLKILTEFSPREPVLGAPELSRRLKIPRTTVFRLLQTLESLGFLERAD
KDRNYRLGVAVLRLGFEYLSSLELTDLGLPIIEALRNETGLTSHIVIRDGRDVVFVAKAQSHAPIFSSVKVNVGTRLPAY
ATTHGQVLMGDLTLDELKKLYPEPELERFTKQTPVTVDDLYERICDDARRGFAISESSFERGISVVSVPVRNDTGRIVAV
ITTTIPRHEIDASLLDSGLIDKVRRAADELSQRLNYRPKAGAKSGSNYMKALGL

Nucleotide

• Length: 885 bp

>AT154365 NZ_CP068033:3933001-3933375 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG