Detailed information of TA system TA154362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1925973..1926538
• Replicon: chromosome
• Accession: NZ_CP051514
• Organism: Paraburkholderia aromaticivorans strain AR20-38

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HF916_RS09040
• Protein ID: WP_168788570.1
• Coordinates: 1925973..1926344 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A242MMY0
• Locus tag: HF916_RS09045
• Protein ID: WP_046565138.1
• Coordinates: 1926341..1926538 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF916_RS09035	1923256..1925484	+	2229	WP_168788569.1	cyclic nucleotide-binding domain-containing protein	-
HF916_RS09040	1925973..1926344	-	372	WP_168788570.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF916_RS09045	1926341..1926538	-	198	WP_046565138.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HF916_RS09050	1927005..1927625	+	621	WP_168788571.1	cytochrome b	-
HF916_RS49860	1927681..1927803	+	123	Protein_1798	VIT family protein	-
HF916_RS09060	1927956..1929101	-	1146	WP_168788572.1	alpha-hydroxy-acid oxidizing protein	-
HF916_RS09065	1929183..1930481	-	1299	WP_168788573.1	L-fuconate dehydratase	-
HF916_RS09070	1930672..1931382	+	711	WP_168788574.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13567.74 Da
• Isoelectric Point: 5.1044

>T154362 WP_168788570.1 NZ_CP051514:c1926344-1925973 [Paraburkholderia aromaticivorans]
MILVDTSVWIDHISASDPMLITLLAQERVLAHPYVIGEISLGSLRDRDVVLGALLDLPRAPVATPEETFYLIDHEGLFNR
GIGYVDTSLLASARLQPGITIWTRDKRLKKVADELNLGALLAH

Nucleotide

• Length: 372 bp

>T154362 NZ_CP068033:358539-358886 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7074.13 Da
• Isoelectric Point: 10.3509

>AT154362 WP_046565138.1 NZ_CP051514:c1926538-1926341 [Paraburkholderia aromaticivorans]
MRTTIALDDDLIAKAQAYTGVEEKTALVREALKALIQREAAKRLANLGGSQPGIKGAPRRRQDVE

Nucleotide

• Length: 198 bp

>AT154362 NZ_CP068033:358886-359185 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A242MMY0