Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 111330..111855 Replicon plasmid pM297-1.2
Accession NZ_CP051492
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HGK25_RS27585 Protein ID WP_013023785.1
Coordinates 111330..111635 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HGK25_RS27590 Protein ID WP_001568025.1
Coordinates 111637..111855 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS27555 (HGK25_28165) 107057..107683 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HGK25_RS28615 107680..107982 + 303 WP_004197636.1 hypothetical protein -
HGK25_RS27565 (HGK25_28175) 108406..109200 - 795 WP_004197635.1 site-specific integrase -
HGK25_RS27570 (HGK25_28185) 109398..110414 - 1017 WP_017899884.1 hypothetical protein -
HGK25_RS27575 (HGK25_28190) 110425..110739 - 315 WP_053389906.1 hypothetical protein -
HGK25_RS27580 (HGK25_28195) 110766..111161 - 396 WP_017899885.1 hypothetical protein -
HGK25_RS27585 (HGK25_28200) 111330..111635 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HGK25_RS27590 (HGK25_28205) 111637..111855 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HGK25_RS27595 (HGK25_28210) 112673..112963 - 291 WP_013023783.1 hypothetical protein -
HGK25_RS27600 (HGK25_28215) 112960..114087 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
HGK25_RS27605 (HGK25_28220) 114121..115644 - 1524 WP_017899887.1 hypothetical protein -
HGK25_RS27610 (HGK25_28225) 115920..116699 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 / blaLAP-2 / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / floR / tet(A) / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..225763 225763


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T154325 WP_013023785.1 NZ_CP051492:c111635-111330 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T154325 NZ_CP068015:2084952-2085054 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT154325 WP_001568025.1 NZ_CP051492:c111855-111637 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT154325 NZ_CP068015:c2085061-2084916 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References