Detailed information of TA system TA154323

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5158938..5159595
• Replicon: chromosome
• Accession: NZ_CP051490
• Organism: Klebsiella pneumoniae strain M297-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HGK25_RS24990
• Protein ID: WP_040245400.1
• Coordinates: 5158938..5159348 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: HGK25_RS24995
• Protein ID: WP_002916312.1
• Coordinates: 5159329..5159595 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGK25_RS24970 (HGK25_25470)	5154938..5156671	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
HGK25_RS24975 (HGK25_25475)	5156677..5157390	-	714	WP_048290928.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HGK25_RS24980 (HGK25_25480)	5157413..5158309	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
HGK25_RS24985 (HGK25_25485)	5158410..5158931	+	522	WP_002916308.1	flavodoxin FldB	-
HGK25_RS24990 (HGK25_25490)	5158938..5159348	-	411	WP_040245400.1	protein YgfX	Toxin
HGK25_RS24995 (HGK25_25495)	5159329..5159595	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
HGK25_RS25000 (HGK25_25500)	5159841..5160824	+	984	WP_065888470.1	tRNA-modifying protein YgfZ	-
HGK25_RS25005 (HGK25_25505)	5160975..5161634	-	660	WP_048334397.1	hemolysin III family protein	-
HGK25_RS25010 (HGK25_25510)	5161798..5162109	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
HGK25_RS25015 (HGK25_25515)	5162159..5162887	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
HGK25_RS25020 (HGK25_25520)	5163006..5164439	+	1434	WP_065888469.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16035.83 Da
• Isoelectric Point: 11.4778

>T154323 WP_040245400.1 NZ_CP051490:c5159348-5158938 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDSRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T154323 NZ_CP068015:734943-735428 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT154323 WP_002916312.1 NZ_CP051490:c5159595-5159329 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT154323 NZ_CP068015:734653-734946 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41