Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 3302120..3303708 Replicon chromosome
Accession NZ_CP051490
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name hipA Uniprot ID A6T928
Locus tag HGK25_RS15950 Protein ID WP_004176197.1
Coordinates 3302386..3303708 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag HGK25_RS15945 Protein ID WP_002904790.1
Coordinates 3302120..3302386 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS15920 (HGK25_16220) 3297271..3298194 + 924 WP_065888692.1 LysR family hpxDE operon transcriptional regulator HpxR -
HGK25_RS15925 (HGK25_16225) 3298175..3299329 - 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
HGK25_RS15930 (HGK25_16230) 3299675..3301054 + 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
HGK25_RS15935 (HGK25_16235) 3301032..3301532 + 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
HGK25_RS15940 (HGK25_16240) 3301529..3301855 + 327 WP_002904788.1 hydroxyisourate hydrolase -
HGK25_RS15945 (HGK25_16245) 3302120..3302386 + 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HGK25_RS15950 (HGK25_16250) 3302386..3303708 + 1323 WP_004176197.1 type II toxin-antitoxin system HipA family toxin Toxin
HGK25_RS15955 (HGK25_16255) 3303725..3305017 - 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
HGK25_RS15960 (HGK25_16260) 3305159..3306163 - 1005 WP_004184029.1 fimbrial protein -
HGK25_RS15965 (HGK25_16265) 3306151..3308652 - 2502 WP_065888691.1 fimbria/pilus outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49111.41 Da        Isoelectric Point: 7.5357

>T154314 WP_004176197.1 NZ_CP051490:3302386-3303708 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T154314 NZ_CP067994:3652613-3652891 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT154314 WP_002904790.1 NZ_CP051490:3302120-3302386 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT154314 NZ_CP067994:3652350-3652610 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6T928

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References