Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1930486..1931105 Replicon chromosome
Accession NZ_CP051490
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HGK25_RS09280 Protein ID WP_002892050.1
Coordinates 1930486..1930704 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HGK25_RS09285 Protein ID WP_002892066.1
Coordinates 1930731..1931105 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS09245 (HGK25_09435) 1926533..1926796 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
HGK25_RS09250 (HGK25_09440) 1926796..1926936 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HGK25_RS09255 (HGK25_09445) 1926933..1927631 - 699 WP_002892021.1 GNAT family protein -
HGK25_RS09260 (HGK25_09450) 1927732..1929183 + 1452 WP_065888842.1 PLP-dependent aminotransferase family protein -
HGK25_RS09265 (HGK25_09455) 1929158..1929628 - 471 WP_002892026.1 YlaC family protein -
HGK25_RS09270 (HGK25_09460) 1929649..1929789 + 141 WP_032409038.1 hypothetical protein -
HGK25_RS09275 (HGK25_09465) 1929761..1930327 - 567 WP_065888843.1 maltose O-acetyltransferase -
HGK25_RS09280 (HGK25_09470) 1930486..1930704 - 219 WP_002892050.1 HHA domain-containing protein Toxin
HGK25_RS09285 (HGK25_09475) 1930731..1931105 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HGK25_RS09290 (HGK25_09480) 1931591..1934737 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HGK25_RS09295 (HGK25_09485) 1934760..1935953 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T154312 WP_002892050.1 NZ_CP051490:c1930704-1930486 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T154312 NZ_CP067994:c3580196-3579855 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT154312 WP_002892066.1 NZ_CP051490:c1931105-1930731 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT154312 NZ_CP067994:c3580534-3580217 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References