Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 732161..732786 Replicon chromosome
Accession NZ_CP051490
Organism Klebsiella pneumoniae strain M297-1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HGK25_RS03585 Protein ID WP_062921072.1
Coordinates 732403..732786 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HGK25_RS03580 Protein ID WP_004150355.1
Coordinates 732161..732403 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGK25_RS03555 (HGK25_03640) 728152..728751 + 600 WP_004151865.1 glucose-1-phosphatase -
HGK25_RS03560 (HGK25_03645) 728745..729605 + 861 WP_004146232.1 virulence factor BrkB family protein -
HGK25_RS03565 (HGK25_03650) 729602..730039 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HGK25_RS03570 (HGK25_03655) 730084..731025 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HGK25_RS03575 (HGK25_03660) 731039..731956 - 918 WP_065888547.1 alpha/beta hydrolase -
HGK25_RS03580 (HGK25_03665) 732161..732403 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HGK25_RS03585 (HGK25_03670) 732403..732786 + 384 WP_062921072.1 type II toxin-antitoxin system VapC family toxin Toxin
HGK25_RS03590 (HGK25_03675) 732960..733889 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HGK25_RS03595 (HGK25_03680) 733886..734521 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HGK25_RS03600 (HGK25_03685) 734518..735420 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T154309 WP_062921072.1 NZ_CP051490:732403-732786 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T154309 NZ_CP067994:2616240-2616347 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT154309 WP_004150355.1 NZ_CP051490:732161-732403 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT154309 NZ_CP067994:c2616193-2616127 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References