Detailed information of TA system TA154307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 53534..54444
• Replicon: plasmid pwem22
• Accession: NZ_CP051489
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HF650_RS24525
• Protein ID: WP_187802924.1
• Coordinates: 53534..54004 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HF650_RS24530
• Protein ID: WP_187802925.1
• Coordinates: 54001..54444 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS24515	49999..51037	+	1039	Protein_46	transposase	-
HF650_RS24520	52445..53443	+	999	WP_187802923.1	tyrosine-type recombinase/integrase	-
HF650_RS24525	53534..54004	-	471	WP_187802924.1	RES family NAD+ phosphorylase	Toxin
HF650_RS24530	54001..54444	-	444	WP_187802925.1	DUF2384 domain-containing protein	Antitoxin
HF650_RS24535	55942..56379	+	438	WP_187802926.1	VOC family protein	-
HF650_RS24540	56352..56805	+	454	Protein_51	Tn3 family transposase	-
HF650_RS24545	57192..58427	+	1236	WP_187802927.1	DUF2254 domain-containing protein	-
HF650_RS24550	58606..58893	+	288	WP_187802928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157340	157340
	-	flank	IS/Tn	-	-	50285..51037	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17552.98 Da
• Isoelectric Point: 5.5836

>T154307 WP_187802924.1 NZ_CP051489:c54004-53534 [Kosakonia sp. SMBL-WEM22]
MILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGTSMVYVSDVASLTMLETLVHLHASHIMDYFTLLRIDVPDEQIQKADMG
ELPGNWADEEAPSELAAYGDAWCFTGSSIALRVPSALSPVEFNYLLNPEHPEFSSVIKHAEEIPFRFDKRLKRARP

Nucleotide

• Length: 471 bp

>T154307 NZ_CP067994:c2377973-2377797 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16455.89 Da
• Isoelectric Point: 9.8472

>AT154307 WP_187802925.1 NZ_CP051489:c54444-54001 [Kosakonia sp. SMBL-WEM22]
MKTFNFSTIQARPPRLWQVAGLNTSDGVALVGQINDGLDGRVATVIADWAKITQNDLRKMSGIPPTTYGRGLKARFSPDQ
SERLVRIIRVIDRAVDLFEGDKEEAQKWLKEPNRALGWKVPAELMASETGAYEVMKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT154307 NZ_CP067994:c2377751-2377335 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA