Detailed information of TA system TA154306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 38215..38852
• Replicon: plasmid pwem22
• Accession: NZ_CP051489
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HF650_RS24460
• Protein ID: WP_187802914.1
• Coordinates: 38670..38852 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HF650_RS24455
• Protein ID: WP_187802913.1
• Coordinates: 38215..38622 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS24425	34140..34502	+	363	WP_187802910.1	hypothetical protein	-
HF650_RS24430	34617..34880	-	264	WP_187802940.1	hypothetical protein	-
HF650_RS24435	34908..35735	-	828	WP_187802911.1	ParA family protein	-
HF650_RS24440	35989..36412	+	424	Protein_31	DUF4158 domain-containing protein	-
HF650_RS24445	36498..37304	-	807	WP_187802912.1	DUF4263 domain-containing protein	-
HF650_RS24450	37611..37897	+	287	Protein_33	transposase	-
HF650_RS24455	38215..38622	-	408	WP_187802913.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HF650_RS24460	38670..38852	-	183	WP_187802914.1	type II toxin-antitoxin system HicA family toxin	Toxin
HF650_RS24465	39566..40153	-	588	Protein_36	recombinase family protein	-
HF650_RS24470	40644..41075	-	432	WP_187802915.1	hypothetical protein	-
HF650_RS24475	41523..42302	+	780	WP_187802916.1	conjugal transfer protein TraX	-
HF650_RS24480	42380..42568	+	189	WP_187802917.1	hypothetical protein	-
HF650_RS24485	42666..42983	+	318	WP_187802918.1	hypothetical protein	-
HF650_RS24490	42973..43776	-	804	WP_187802919.1	DUF4225 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..157340	157340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6663.79 Da
• Isoelectric Point: 10.4971

>T154306 WP_187802914.1 NZ_CP051489:c38852-38670 [Kosakonia sp. SMBL-WEM22]
MSSGELIKKLTQAGWVHVRTKGSHHIFEKDGELNPKVVPHPTKDLAVGTLHKILKQLGEK

Nucleotide

• Length: 183 bp

>T154306 NZ_CP067994:2295086-2296408 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14904.47 Da
• Isoelectric Point: 4.4142

>AT154306 WP_187802913.1 NZ_CP051489:c38622-38215 [Kosakonia sp. SMBL-WEM22]
MKYPVYLHQAESGTFSGFVPDIAGCYFAGDNIDEAIADAHAAIDAYLEYQSEKGREVPDAGTINDYKDDEDCRGGIWGLV
DIDTSKYQGKAVKLNITLPQNLLSRIDTYVSSNGDYTSRSGFLAELARRELLKHS

Nucleotide

• Length: 408 bp

>AT154306 NZ_CP067994:2294820-2295086 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA