Detailed information of TA system TA154304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 4280127..4280646
• Replicon: chromosome
• Accession: NZ_CP051488
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0MI25
• Locus tag: HF650_RS20530
• Protein ID: WP_042717987.1
• Coordinates: 4280365..4280646 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: HF650_RS20525
• Protein ID: WP_042717989.1
• Coordinates: 4280127..4280375 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS20510	4275892..4277151	-	1260	WP_187800154.1	oligosaccharide MFS transporter	-
HF650_RS20515	4277209..4279035	-	1827	WP_187800155.1	DUF2264 domain-containing protein	-
HF650_RS20520	4279201..4280076	+	876	WP_187800156.1	AraC family transcriptional regulator	-
HF650_RS20525	4280127..4280375	+	249	WP_042717989.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HF650_RS20530	4280365..4280646	+	282	WP_042717987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF650_RS20535	4280817..4282709	-	1893	WP_187800157.1	DNA topoisomerase IV subunit B	-
HF650_RS20540	4282749..4283324	-	576	WP_187800158.1	esterase YqiA	-
HF650_RS20545	4283324..4284151	-	828	WP_187800159.1	3',5'-cyclic-AMP phosphodiesterase	-
HF650_RS20550	4284176..4284598	-	423	WP_023480711.1	DUF1249 family protein	-
HF650_RS20555	4284595..4285227	-	633	WP_187800160.1	ADP-ribose diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10914.71 Da
• Isoelectric Point: 10.6500

>T154304 WP_042717987.1 NZ_CP051488:4280365-4280646 [Kosakonia sp. SMBL-WEM22]
MTYKLAFNVSALKEWKKLGSTVREQFKKKLQERLENPRVPAAQLHGRKDQYKIKLRGAGYRLVYKVEDEIITVTVIGVGK
RENDEIYTATRFR

Nucleotide

• Length: 282 bp

>T154304 NZ_CP067994:1961369-1961472 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9097.37 Da
• Isoelectric Point: 3.9490

>AT154304 WP_042717989.1 NZ_CP051488:4280127-4280375 [Kosakonia sp. SMBL-WEM22]
MPFQILTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYARLMEMLEDEELGRIADERADEPTIEVKLD
DL

Nucleotide

• Length: 249 bp

>AT154304 NZ_CP067994:c1961510-1961239 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A7H0MI25