Detailed information of TA system TA154303

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4133846..4134509
• Replicon: chromosome
• Accession: NZ_CP051488
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HF650_RS19855
• Protein ID: WP_187800052.1
• Coordinates: 4133846..4134262 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A807LAC0
• Locus tag: HF650_RS19860
• Protein ID: WP_023480982.1
• Coordinates: 4134243..4134509 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS19835	4129837..4131570	-	1734	WP_187800049.1	single-stranded-DNA-specific exonuclease RecJ	-
HF650_RS19840	4131576..4132286	-	711	WP_042718296.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HF650_RS19845	4132311..4133207	-	897	WP_187800050.1	site-specific tyrosine recombinase XerD	-
HF650_RS19850	4133307..4133828	+	522	WP_187800051.1	flavodoxin FldB	-
HF650_RS19855	4133846..4134262	-	417	WP_187800052.1	protein YgfX	Toxin
HF650_RS19860	4134243..4134509	-	267	WP_023480982.1	FAD assembly factor SdhE	Antitoxin
HF650_RS19865	4134757..4135737	+	981	WP_187800053.1	tRNA-modifying protein YgfZ	-
HF650_RS19870	4135787..4136446	-	660	WP_187800054.1	hemolysin III family protein	-
HF650_RS19875	4136673..4137401	+	729	WP_023481032.1	MurR/RpiR family transcriptional regulator	-
HF650_RS19880	4137518..4138951	+	1434	WP_187800055.1	6-phospho-beta-glucosidase	-
HF650_RS19885	4138993..4139265	+	273	WP_187800056.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16386.31 Da
• Isoelectric Point: 10.7346

>T154303 WP_187800052.1 NZ_CP051488:c4134262-4133846 [Kosakonia sp. SMBL-WEM22]
VVLWQSELRVSWRAQWLSLLLHGLVAALILLMPWPLSYTPVWLLLLSLVVFDSVRSQRRINACHGEIKLLMDSRLRWQGE
EWDIVGTPWLLRSGMMLRLRREEDGRRQHLWLAADSMDAQEWRDLRRMILQKPAQGLH

Nucleotide

• Length: 417 bp

>T154303 NZ_CP067994:c1806586-1806212 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10556.01 Da
• Isoelectric Point: 5.6186

>AT154303 WP_023480982.1 NZ_CP051488:c4134509-4134243 [Kosakonia sp. SMBL-WEM22]
MDITNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDDKRLFVRLLENDDPDLFNWLMNHGKPQDAELQRMVQLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT154303 NZ_CP067994:c1807043-1806675 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A807LAC0