Detailed information of TA system TA154302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3040116..3041026
• Replicon: chromosome
• Accession: NZ_CP051488
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HF650_RS14585
• Protein ID: WP_187799278.1
• Coordinates: 3040116..3040586 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HF650_RS14590
• Protein ID: WP_187799279.1
• Coordinates: 3040583..3041026 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS14565	3037187..3037426	-	240	WP_187799276.1	histidine kinase	-
HF650_RS14570	3038293..3038562	-	270	WP_187799277.1	hypothetical protein	-
HF650_RS14575	3038908..3039288	-	381	Protein_2866	DUF2778 domain-containing protein	-
HF650_RS14580	3039866..3040033	+	168	Protein_2867	hypothetical protein	-
HF650_RS14585	3040116..3040586	-	471	WP_187799278.1	RES family NAD+ phosphorylase	Toxin
HF650_RS14590	3040583..3041026	-	444	WP_187799279.1	DUF2384 domain-containing protein	Antitoxin
HF650_RS14595	3041245..3041418	-	174	WP_187799280.1	hypothetical protein	-
HF650_RS14600	3041617..3041940	+	324	WP_187799281.1	hypothetical protein	-
HF650_RS14605	3042293..3043122	+	830	Protein_2872	transposase	-
HF650_RS14610	3043217..3043486	-	270	WP_187799282.1	MarR family transcriptional regulator	-
HF650_RS14615	3043885..3044253	+	369	WP_187799283.1	DUF2778 domain-containing protein	-
HF650_RS14620	3044250..3044597	+	348	WP_187799284.1	hypothetical protein	-
HF650_RS14625	3045052..3045408	-	357	WP_187802702.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3012978..3052936	39958
	-	flank	IS/Tn	-	-	3042293..3042544	251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17842.31 Da
• Isoelectric Point: 4.9881

>T154302 WP_187799278.1 NZ_CP051488:c3040586-3040116 [Kosakonia sp. SMBL-WEM22]
MILYRLTKTKYLSTAWTGYGAKEAGGRWNNVGTAMVYVSEAASLTMLETLVHLRATHIMDYFTLLRIDVPDVQIQKANMD
ELPANWADEEAPPELAAYGDAWCFTGSSIALRVPSALSPVEFNYLLNPEHPDYYRVIKDSEEIPFRFDSRLKRERF

Nucleotide

• Length: 471 bp

>T154302 NZ_CP067994:1794690-1794944 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16455.99 Da
• Isoelectric Point: 9.8933

>AT154302 WP_187799279.1 NZ_CP051488:c3041026-3040583 [Kosakonia sp. SMBL-WEM22]
MKTFNLSTTQAKPPRLWQVAGLNTSDGVALVGQINEGLDGKVATLIVDWAKMSQNDLRKMSGIPPTTFGRSLKARFTPDQ
SERLVRIIRVIDRAVELFEGDKEEAQKWLKEPNRALGWKVPAELMASETGAYEVMKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT154302 NZ_CP067994:1794442-1794693 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA