Detailed information of TA system TA154293

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1432358..1433025
• Replicon: chromosome
• Accession: NZ_CP051488
• Organism: Kosakonia sp. SMBL-WEM22

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HF650_RS06825
• Protein ID: WP_187801732.1
• Coordinates: 1432621..1433025 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HF650_RS06820
• Protein ID: WP_187801731.1
• Coordinates: 1432358..1432621 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF650_RS06805	1428225..1429643	-	1419	WP_187801729.1	melibiose:sodium transporter MelB	-
HF650_RS06810	1429714..1431066	-	1353	WP_187801730.1	alpha-glucosidase/alpha-galactosidase	-
HF650_RS06815	1431301..1432218	+	918	WP_042715696.1	transcriptional regulator MelR	-
HF650_RS06820	1432358..1432621	+	264	WP_187801731.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HF650_RS06825	1432621..1433025	+	405	WP_187801732.1	HipA N-terminal domain-containing protein	Toxin
HF650_RS06830	1433163..1433615	-	453	WP_187801733.1	DUF1311 domain-containing protein	-
HF650_RS06835	1433612..1434535	-	924	WP_187801734.1	peptidoglycan-binding protein	-
HF650_RS06840	1434796..1436334	-	1539	WP_187801735.1	MCP four helix bundle domain-containing protein	-
HF650_RS06845	1436765..1437082	-	318	WP_187801736.1	hypothetical protein	-
HF650_RS06850	1437326..1437499	+	174	WP_187801737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-67)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15186.11 Da
• Isoelectric Point: 6.9905

>T154293 WP_187801732.1 NZ_CP051488:1432621-1433025 [Kosakonia sp. SMBL-WEM22]
MRKLSTWMNNERVGELTKQSNGAHTFKYDEGWLQSERARPLSLSLPLQFGAITSDAVFNYFDNLLPDNPRVRDRIVKRYH
AASKQPFDLLAEIGRDSVGAVTLLPPEEESASRALSWAMLEQAKHSLPDNIHHA

Nucleotide

• Length: 405 bp

>T154293 NZ_CP067994:184942-185049 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9927.38 Da
• Isoelectric Point: 7.0091

>AT154293 WP_187801731.1 NZ_CP051488:1432358-1432621 [Kosakonia sp. SMBL-WEM22]
MTTPAIYSPVQLANAMKLIRQKNGWTQNDLAQRIGIKQATISNFENNPDKTTLTTLFKILQSLEMSLILQEKKEAGVDNL
HQQDLEW

Nucleotide

• Length: 264 bp

>AT154293 NZ_CP067994:c184893-184827 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC