Detailed information of TA system TA154281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1447030..1447544
• Replicon: chromosome
• Accession: NZ_CP051484
• Organism: Staphylococcus aureus strain NGA71

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: HGO96_RS07150
• Protein ID: WP_054190442.1
• Coordinates: 1447030..1447239 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: HGO96_RS07155
• Protein ID: WP_000138934.1
• Coordinates: 1447251..1447544 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO96_RS07130 (1442538)	1442538..1443896	-	1359	WP_054190439.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
HGO96_RS07135 (1443911)	1443911..1444981	-	1071	WP_054190440.1	D-alanine--D-alanine ligase	-
HGO96_RS07140 (1445299)	1445299..1446501	+	1203	WP_054190441.1	FtsW/RodA/SpoVE family cell cycle protein	-
HGO96_RS07145 (1446765)	1446765..1446902	-	138	WP_000828354.1	Lmo0850 family protein	-
HGO96_RS07150 (1447030)	1447030..1447239	-	210	WP_054190442.1	heavy metal-associated domain-containing protein	Toxin
HGO96_RS07155 (1447251)	1447251..1447544	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
HGO96_RS07160 (1447714)	1447714..1449198	+	1485	WP_054190443.1	cardiolipin synthase	-
HGO96_RS07165 (1449226)	1449226..1449873	+	648	WP_001187616.1	HD domain-containing protein	-
HGO96_RS07170 (1450453)	1450453..1451325	-	873	WP_000725800.1	membrane protein insertase YidC	-
HGO96_RS07175 (1451411)	1451411..1452052	-	642	WP_054190444.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7830.77 Da
• Isoelectric Point: 4.1008

>T154281 WP_054190442.1 NZ_CP051484:c1447239-1447030 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISSMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKILF

Nucleotide

• Length: 210 bp

>T154281 NZ_CP067966:c1801552-1801199 [Klebsiella quasipneumoniae]
ATGTGGACAATTAAAACAACGGATACGTTTGATCGCTGGTTTGCCTCGCTCAGCGATACCGACAGGGTCAGCATGCTTGC
AGCACTGCTGGTCCTGCGGGAAAAGGGACCAGGTTTGTCCAGACCTTATGCCGATACGGTCAGAGGTTCCCGCTACAGCA
ACATGAAAGAGCTACGTATTCAGAGTCGGGGCGATCCGATACGGGCGTTTTTCGCTTTTGACCCGACCCGTACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGACAGGGAATTCAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT154281 WP_000138934.1 NZ_CP051484:c1447544-1447251 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT154281 NZ_CP067966:c1801194-1800892 [Klebsiella quasipneumoniae]
GTGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAATCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCAACGGACAT
ACTGCTCAACATTCACCTTGCCGAACTGCGTGAGAAAGTACAGAAAACGCAGGTGGAGATGGCACAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACCAGGGCGCGATCTGAAGCTTTCCACTCTCAAACGCTACGTTGAAGCGACT
GGCGGCAAGCTACGTCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTTGTACTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0D1GBN9