Detailed information of TA system TA154280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1427289..1427818
• Replicon: chromosome
• Accession: NZ_CP051484
• Organism: Staphylococcus aureus strain NGA71

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HGO96_RS07060
• Protein ID: WP_000621175.1
• Coordinates: 1427289..1427651 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HGO96_RS07065
• Protein ID: WP_000948331.1
• Coordinates: 1427648..1427818 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO96_RS07040 (1424267)	1424267..1425037	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
HGO96_RS07045 (1425012)	1425012..1425491	-	480	WP_054190427.1	anti-sigma B factor RsbW	-
HGO96_RS07050 (1425493)	1425493..1425819	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HGO96_RS07055 (1425938)	1425938..1426939	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HGO96_RS07060 (1427289)	1427289..1427651	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGO96_RS07065 (1427648)	1427648..1427818	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HGO96_RS07070 (1427903)	1427903..1429051	-	1149	WP_054190429.1	alanine racemase	-
HGO96_RS07075 (1429117)	1429117..1429476	-	360	WP_000581197.1	holo-ACP synthase	-
HGO96_RS07080 (1429480)	1429480..1429971	-	492	WP_054190430.1	PH domain-containing protein	-
HGO96_RS07085 (1429958)	1429958..1431541	-	1584	WP_054190431.1	PH domain-containing protein	-
HGO96_RS07090 (1431534)	1431534..1432013	-	480	WP_054190432.1	hypothetical protein	-
HGO96_RS07095 (1432222)	1432222..1432782	-	561	WP_054190433.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T154280 WP_000621175.1 NZ_CP051484:c1427651-1427289 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T154280 NZ_CP051484:c1427651-1427289 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT154280 WP_000948331.1 NZ_CP051484:c1427818-1427648 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT154280 NZ_CP051484:c1427818-1427648 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1