Detailed information of TA system TA154267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2722018..2722547
• Replicon: chromosome
• Accession: NZ_CP051483
• Organism: Staphylococcus aureus strain NGA66a

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HGO94_RS13455
• Protein ID: WP_000621175.1
• Coordinates: 2722185..2722547 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HGO94_RS13450
• Protein ID: WP_000948331.1
• Coordinates: 2722018..2722188 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO94_RS13420 (2717054)	2717054..2717614	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
HGO94_RS13425 (2717823)	2717823..2718302	+	480	WP_001287078.1	hypothetical protein	-
HGO94_RS13430 (2718295)	2718295..2719878	+	1584	WP_001294626.1	PH domain-containing protein	-
HGO94_RS13435 (2719865)	2719865..2720356	+	492	WP_072519462.1	PH domain-containing protein	-
HGO94_RS13440 (2720360)	2720360..2720719	+	360	WP_000581197.1	holo-ACP synthase	-
HGO94_RS13445 (2720785)	2720785..2721933	+	1149	WP_001281154.1	alanine racemase	-
HGO94_RS13450 (2722018)	2722018..2722188	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HGO94_RS13455 (2722185)	2722185..2722547	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGO94_RS13460 (2722897)	2722897..2723898	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HGO94_RS13465 (2724017)	2724017..2724343	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HGO94_RS13470 (2724345)	2724345..2724824	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
HGO94_RS13475 (2724799)	2724799..2725569	+	771	WP_001041108.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T154267 WP_000621175.1 NZ_CP051483:2722185-2722547 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T154267 NZ_CP067426:3436885-3437172 [Escherichia coli O22:H8]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGGTGTGTGCTGCCGTTATCCGAACACCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCTCACCCTAACTATATAATTCTTTACCGTGTAGCCACTTTAAGCATTGAG
ATCGTGAGTGTAGTACACGCCAGACGCCAATTCCCCAAGCAGAACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT154267 WP_000948331.1 NZ_CP051483:2722018-2722188 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT154267 NZ_CP067426:3436693-3436881 [Escherichia coli O22:H8]
ATGAATACGGCCCTTTCACCAATGGTTTCTGAGTTCGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAAATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1