Detailed information of TA system TA154250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2763922..2764451
• Replicon: chromosome
• Accession: NZ_CP051482
• Organism: Staphylococcus aureus strain NGA104a

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HGO93_RS13635
• Protein ID: WP_000621175.1
• Coordinates: 2764089..2764451 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HGO93_RS13630
• Protein ID: WP_000948331.1
• Coordinates: 2763922..2764092 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGO93_RS13600 (2758958)	2758958..2759518	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
HGO93_RS13605 (2759727)	2759727..2760206	+	480	WP_001287078.1	hypothetical protein	-
HGO93_RS13610 (2760199)	2760199..2761782	+	1584	WP_001294626.1	PH domain-containing protein	-
HGO93_RS13615 (2761769)	2761769..2762260	+	492	WP_072519462.1	PH domain-containing protein	-
HGO93_RS13620 (2762264)	2762264..2762623	+	360	WP_000581197.1	holo-ACP synthase	-
HGO93_RS13625 (2762689)	2762689..2763837	+	1149	WP_001281154.1	alanine racemase	-
HGO93_RS13630 (2763922)	2763922..2764092	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HGO93_RS13635 (2764089)	2764089..2764451	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HGO93_RS13640 (2764801)	2764801..2765802	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HGO93_RS13645 (2765921)	2765921..2766247	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HGO93_RS13650 (2766249)	2766249..2766728	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
HGO93_RS13655 (2766703)	2766703..2767473	+	771	WP_001041108.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T154250 WP_000621175.1 NZ_CP051482:2764089-2764451 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T154250 NZ_CP067420:5322116-5322385 [Skermanella sp. TT6]
TTGACGAGACTCGCCTGGACCGACGCTGCTTGGGAAGATTATACATGGTGGCAGGAGAACGATCGCAAGACGGCCCGCAG
GATCAACGAGTTGATCAAGAGCACCTTGCGAACGCCCTTCGATGGCGTTGGGAAACCTGAGCCATTGAGAGGGGATTGGG
CGGGGTGGTGGTCTCGGCGGATCACCTTGGAACACCGCTTGGTATACCGAAACTTTGGTAACGGCGATGACGAGACATTG
ATCATCGCCCAGTGCCGTTATCATTACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT154250 WP_000948331.1 NZ_CP051482:2763922-2764092 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT154250 NZ_CP067420:5321817-5322119 [Skermanella sp. TT6]
ATGGGACACGTCAGCTATACTGAACTTCGGCAAAATCTGGCCCGGTACATGGATGAAGTATGCGACAGCAATGCGCCCCT
CGTCGTCACACGGCAGAATGCGCGCCCTGTCGTGATGATCAGCCAGGATGAGTACGAGAGCATGGCGGCAACTCTCCACC
TGATCAAGTCGCCTCGCAACGCCCTTCGGCTCATCGAGTCCATGGAGGACGCCAAAGCTGGCCGGGTAAGCGAACGGGGA
TTGATAGAGGTGGACGACGAGGCGGAAACCGACCTTCGCACCGGCGATGGTGAGCCGGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1