Detailed information of TA system TA154214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-doc
• Location: 114143..114755
• Replicon: plasmid pRspCH10DE
• Accession: NZ_CP051473
• Organism: Cereibacter sphaeroides strain CH10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q3IUZ4
• Locus tag: HGN32_RS22235
• Protein ID: WP_011331447.1
• Coordinates: 114360..114755 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IUZ5
• Locus tag: HGN32_RS22230
• Protein ID: WP_011331446.1
• Coordinates: 114143..114370 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGN32_RS22215	110364..111575	-	1212	WP_011331443.1	replication initiation protein RepC	-
HGN32_RS22220	111758..112744	-	987	WP_011331444.1	plasmid partitioning protein RepB	-
HGN32_RS22225	112744..113928	-	1185	WP_011331445.1	plasmid partitioning protein RepA	-
HGN32_RS22230	114143..114370	+	228	WP_011331446.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HGN32_RS22235	114360..114755	+	396	WP_011331447.1	PIN domain-containing protein	Toxin
HGN32_RS22240	114835..115718	-	884	Protein_116	recombinase family protein	-
HGN32_RS22245	116431..117024	+	594	WP_011331449.1	biliverdin-producing heme oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..152929	152929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• SpoVT-AbrB domain (5-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14296.31 Da
• Isoelectric Point: 4.3422

>T154214 WP_011331447.1 NZ_CP051473:114360-114755 [Cereibacter sphaeroides]
MSAEFADTNVILYLLDNGPKADCAEEILGHRPRISVQVLNETLVNCRRKAGLSWEEAGAFLEGVRALCPVESLTIQTHDV
GRALAERYGFSIYDSIIVASALVAGCTTLWSEDMQDGLLVEGQLRIVNPFA

Nucleotide

• Length: 396 bp

>T154214 NZ_CP067399:c3741137-3740739 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8569.87 Da
• Isoelectric Point: 10.9144

>AT154214 WP_011331446.1 NZ_CP051473:114143-114370 [Cereibacter sphaeroides]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDHQVRVRRLARADEVLMGLRRFRGALPAAERLSRNDAHER

Nucleotide

• Length: 228 bp

>AT154214 NZ_CP067399:c3741433-3741140 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: Q3IUZ4

Antitoxin

• AlphaFold DB: Q3IUZ5