Detailed information of TA system TA154213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 73475..74112
• Replicon: plasmid pRspCH10C
• Accession: NZ_CP051472
• Organism: Cereibacter sphaeroides strain CH10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HGN32_RS21475
• Protein ID: WP_011331362.1
• Coordinates: 73475..73882 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3IV80
• Locus tag: HGN32_RS21480
• Protein ID: WP_011331361.1
• Coordinates: 73882..74112 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGN32_RS21460	70163..70330	-	168	Protein_70	Hsp20/alpha crystallin family protein	-
HGN32_RS21465	70362..72986	-	2625	WP_017140473.1	cation-transporting P-type ATPase	-
HGN32_RS21470	72995..73153	-	159	WP_017140474.1	SDR family oxidoreductase	-
HGN32_RS21475	73475..73882	-	408	WP_011331362.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HGN32_RS21480	73882..74112	-	231	WP_011331361.1	antitoxin	Antitoxin
HGN32_RS21485	74501..75334	-	834	WP_017140476.1	hypothetical protein	-
HGN32_RS21490	75547..75972	-	426	WP_011331359.1	hypothetical protein	-
HGN32_RS21495	76260..76685	+	426	WP_011331358.1	helix-turn-helix transcriptional regulator	-
HGN32_RS21500	77248..78135	+	888	WP_011331357.1	viral aspartic protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd / fcl	1..106879	106879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14801.00 Da
• Isoelectric Point: 6.8714

>T154213 WP_011331362.1 NZ_CP051472:c73882-73475 [Cereibacter sphaeroides]
MLRYMLDTNILIYTIRNRPGEVRAKFEAHHGTMCISAATAMELIYGAQKSQAVERNVAAVEGLLARLDILDLDLAAAQHA
GEIRAQLAQAGTPIGPFDVMIAGHARSKGLVLVSNNTREFERVDGLRLENWVQSS

Nucleotide

• Length: 408 bp

>T154213 NZ_CP067399:3482875-3483093 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8777.77 Da
• Isoelectric Point: 4.5600

>AT154213 WP_011331361.1 NZ_CP051472:c74112-73882 [Cereibacter sphaeroides]
VDRGSVFMNNRTQAIRLPKSVALPEGTRSVDIVRLGRAWLIVPSQDSWAQWFDEVSSSEDFMERRKQPDADERDDL

Nucleotide

• Length: 231 bp

>AT154213 NZ_CP067399:3482475-3482849 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: Q3IV80